[Histonet] Storing frozen TMA sections

Instrumedics info <@t> instrumedics.com
Wed Jun 1 15:02:24 CDT 2005

We coat our blocks with Instrumedcis Protective Oil that has a freezing 
point of -10C. It will not melt the tissue cores in the block and will 
prevent dehydration when storing in a freezer for even long periods.

We also coat the blockface when we leave the block in the microtome for next 
day sectioning The frozen oil can be sectioned, just like embedding media, 
so it can removed and the tissue cores are ready to be sectioned.


----- Original Message ----- 
From: "CHRISTIE GOWAN" <christiegowan <@t> msn.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, June 01, 2005 1:52 PM
Subject: [Histonet] Storing frozen TMA sections

>I know this is an old problem but I was wondering if Patsy Ruegg or Chris 
>Van der Loos or anyone for that matter could send me their protocol for 
>storing frozen TMA sections. Has anyone ever tried to store them in vacuum 
>sealed (Food Saver) bags? I work for a company that provides frozen TMA 
>slides for customers. We section them as needed and have discovered that 
>the tissue starts showing freezer artifact after going into the block about 
>10-12 times. Any help would be greatly appreciated.
> Thanks,
> Christie Gowan

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