[Histonet] Ki67.

[Bruijntjes, J.P.]

Hi Christian

Yes I do. May be it depends on the antibody you use. We use a rabbit
monoclonal ki67 from Neomarkers (Labvision). Tissues fixed in alcohol
don't react with the antibody using HIER in citrate buffer (pH 6,0). At
least it didn't in my lab. It does when you use HIER in EDTA (pH 8.0)
and in TRIS (pH 10). 
Hopes that will help you

Joost Bruijntjes
TNO Quality of Life
Zeist
Holland


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
christian <@t> eisbo.dk
Sent: woensdag 1 juni 2005 9:51
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ki67.

Hey users of histonet.
I am trying to stain kidneys for ki67!
The sections have been in alcohol for 1 month and are now in parafin.
The antibody I am using should be good enough.
Does anyone have experience with this???
best regards. Christian EIsbo.

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