[Histonet] Bone processing

[Elizabeth Chlipala]

Steve

We process mouse joints all of time and use vacuum/pressure during the
processing cycle.  The cycle we use is long approximately 14 + hours.  

50% alcohol - 60 minutes
70% alcohol - 60 minutes
80% alcohol - 60 minutes
95% alcohol - 60 minutes
100% alcohol - 60 minutes
100% alcohol - 60 minutes
100% alcohol - 60 minutes
Xylene - 60 minutes
Xylene - 60 minute
Xylene - 60 minutes
Paraffin - 60 minutes
Paraffin - 60 minutes
Paraffin - 60 minutes
Paraffin - 60 minutes

Liz


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Thursday, July 28, 2005 1:19 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bone processing

New to research, just received the "Animal Processing Manual".  I'm
about to process mouse joints and I noticed a much longer time
especially in the infiltration paraffins.
 
Along these lines I also noticed processing for mouse/rat tissue calls
for only RT processing and no vacuum/pressure..
 
I have a clinical background so some or allot of this is a "tad" new.
Anyone up to explaining the big picture difference between human tissue
verses animal tissue processing.
 
Steve


		
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