[Histonet] ck7 and ck20controls
Clarke, Mary
mclarke <@t> allsaintshealthcare.org
Wed Jul 27 13:21:50 CDT 2005
We use a bladder carcinoma for the ck7 and a mucicarmine control for the
ck20.
Terri Clarke
Histology Supervisor
All Saints Laboratory
3801 Spring Street
Racine, Wi 53405
mclarke <@t> allsaintshealthcare.org
262-687-6609
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, July 26, 2005 4:56 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 36
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Genta stain for Helicobacter pylori (IamNinaOwen <@t> aol.com)
2. stain for pre-collagen and mature collagen (Steven Coakley)
3. re: iNOS (Kristopher Kalleberg)
4. RE: Genta stain for Helicobacter pylori (Monfils, Paul)
5. granular debris in paraffin blocks (Diana McCaig)
6. re: iNOS (Kristopher Kalleberg)
7. Re: Integrase Interactor 1 (INI-1/hsnf5) antibody needed
(Joanne Mauger)
8. Applied IHC Journal (Richard Cartun)
9. RE: granular debris in paraffin blocks (Monfils, Paul)
10. Serotec Rat Anti-mouse F4/80 : Biotin -- anyone know of good
concentration? (GT Hebert)
11. Biotin conjugated Antibodies -- can we skip biotin?? (GT Hebert)
12. anti-mouse cleaved PARP for IHC... (Glenn Krasinski)
13. CK7 control/ CK20 contol (Amy Johnson)
14. RE: CK7 control/ CK20 contol (Sebree Linda A.)
15. Re: Serotec Rat Anti-mouse F4/80 : Biotin -- anyone know of
good concentration? (Gayle Callis)
16. Re: Serotec Rat Anti-mouse F4/80 : Biotin -- anyone know of
good concentration? (GT Hebert)
17. Re: Biotin conjugated Antibodies -- can we skip biotin??
(Gayle Callis)
18. Artifact pictures and cell block prep (Woodward, Denise)
19. Productivity (Dndsomi <@t> aol.com)
20. bunny Ab's (Christian Franci)
21. Re: Artifact pictures and cell block prep (Geoff McAuliffe)
22. RE: Artifact pictures and cell block prep (Monfils, Paul)
23. RE: Artifact pictures and cell block prep (Woodward, Denise)
24. RE: bunny Ab's (Linke_Noelle)
25. un-subscribe (Henry, Charlene)
----------------------------------------------------------------------
Message: 1
Date: Tue, 26 Jul 2005 14:29:31 EDT
From: IamNinaOwen <@t> aol.com
Subject: [Histonet] Genta stain for Helicobacter pylori
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20.4990d7aa.3017db0b <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hello Histonetters!
Has anyone ever used the Genta stain for Helicobacter pylori? I'm
trying to
find a protocol for this method, so any help will be very much
appreciated!
Thanks
Nina Owen
------------------------------
Message: 2
Date: Tue, 26 Jul 2005 11:30:39 -0700 (PDT)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] stain for pre-collagen and mature collagen
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050726183039.46878.qmail <@t> web90204.mail.scd.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I've been working on Herovica's Method for differentiating
pre-collagen(young) verses mature collagen using varies combinations of
methy blue and van gieson's. Luna has a modified method on pg 453 of
his "Histopathologic Methods...." The regular Herovica stain calls for
tissue fixed in formol-acetic-alcohol. 10% NBF leaves the muscle a blue
to blue-green and very little red (mature collagen). I tried post
fixation in the FAA for 1 hr w/o allot of difference.
Bouins post-fixation seems to work best. Certainly not getting the
results as outlined. Is there anyway to check the saturated picric
acid? Any ideas or suggestion would be appreciated, especially from
those familiar with this stain.
Steve
---------------------------------
Start your day with Yahoo! - make it your home page
------------------------------
Message: 3
Date: Tue, 26 Jul 2005 14:29:43 -0400
From: "Kristopher Kalleberg" <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] re: iNOS
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<ISSMTP.2002_3_.20050726142943.2528D <@t> usl-006.na.unilever.com>
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
If I remember right Neomarkers/Lab Vision Corp. has iNOS antibodies. I
used
them maybe 1 1/2 years ago but never got great results from the stain.
I ran
the test numerous times and was unable to solve the problem. If you
decide to
use Neomarkers and are able to achieve good results please send me your
secret.
Good luck.
Kris Kalleberg
Histologist
Unilever R&D
40 Merritt Blvd.
Trumbull, CT 06611
203 381 5765
-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu]
Sent: Tuesday, July 26, 2005 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 35
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. gluteraldehyde fixation with Re: [Histonet] Rat brains
(Gayle Callis)
2. IHC for RE:Staining for Helicobacter pylori (Gayle Callis)
3. Re: Staining for Helicobacter pylori (IamNinaOwen <@t> aol.com)
4. TMB staining for WGA-HRP (NIENHUIS,ROBERT )
5. historesin (Walters, Katherine S)
6. equivalent to Re: [Histonet] historesin (Gayle Callis)
7. Janella Seaton...please contact me (Morken, Tim - Labvision)
8. Background staining: mouse on mouse Broad spectrum kit
(Cathy Malcontenti-Wilson)
9. Re: historesin (Don.Birgerson <@t> leica-microsystems.com)
10. Re: CD 31 (Jackie M O'Connor)
11. North Carolina Histologist Position Available (MajorFocus <@t> aol.com)
12. Re: equivalent to Re: [Histonet] historesin (Pamela Marcum)
13. Looking 4 Antibodies (Luis Chiriboga)
14. DIF procedures (pathrm35 <@t> adelphia.net)
15. teaching heads for Nikon microscope (pathrm35 <@t> adelphia.net)
16. Integrase Interactor 1 (INI-1/hsnf5) antibody needed
(Histology SLU)
17. looking for iNOS antibody (Louro, Pedro)
18. Re: Integrase Interactor 1 (INI-1/hsnf5) antibody needed
(Dana Settembre)
19. IgG2a and IgG1 for guinea pig (Elizabeth Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Mon, 25 Jul 2005 11:21:28 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: gluteraldehyde fixation with Re: [Histonet] Rat brains
To: Laura Fidgen <lfidgen <@t> vt.edu>, Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050725111447.01b7ada0 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Question: is he planning to do EM on these tissues? If he had used
paraformaldehyde, your sectioning would be much easier - but if he is
also
doing EM - you can try some things to counteract the dry/brittle brain.
In general, gluteraldehyde creates some friable, brittle tissues when
these are processed and embedded in paraffin. Check to see if you are
processing too long as long dehydrations, etc and heat from paraffin
contributes to the problem.
A good long soak in ice water or solid ice block with water on top after
trimming might help but be sure to not retrim the soak away, you have to
get the first sections off the knife. A warm water or room temperature
soak the going onto ice water for a time may help too, brain sometimes
needs a bit of help.
At 01:01 PM 7/23/2005, you wrote:
>Please help...
>A research project for our neuropathologist includes rat brains. These
>are extremely difficult to section as he profuses the whole rat with
>glutaraldehyde then takes brain samples. I have tried adding alcohol
to
>my water bath but these sections still have many, many wrinkles. Can
>anyone offer suggestions? I would be so grateful for any and all
advice.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 2
Date: Mon, 25 Jul 2005 11:25:52 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] IHC for RE:Staining for Helicobacter pylori
To: Tom McNemar <TMcNemar <@t> lmhealth.org>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050725112230.01b7b5c8 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Dear Tom,
If I had to do Helicobacter, I would vote for IHC too!!! Very specific
for
identification of Helicobacter in human tissues, I only wished I had it
for
murine animal model work but no antibody was available - boo hoo!!!
There
is a nice method published in Histologic, Sakura Finetek website on
doing
this IHC, and it was wonderful.
At 07:05 AM 7/25/2005, you wrote:
>We have been doing them by IHC for the last few years. Overkill, I
know.
>The paths never like the Giemsa and we had so much trouble with the
>Warthin-Starry (consistency, repeats, etc) that I just switched to IHC.
>Works every time.
>Tom McNemar, HT(ASCP)
>Histology Co-ordinator
>Licking Memorial Hospital
>Newark, Ohio 43055
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 3
Date: Mon, 25 Jul 2005 13:30:46 EDT
From: IamNinaOwen <@t> aol.com
Subject: [Histonet] Re: Staining for Helicobacter pylori
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <65.49f13cd4.30167bc6 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hi all
Thanks for all your views on this matter, keep the replies rolling in!
Nina Owen
------------------------------
Message: 4
Date: Mon, 25 Jul 2005 11:55:40 -0700
From: "NIENHUIS,ROBERT " <nienhuis <@t> ucla.edu>
Subject: [Histonet] TMB staining for WGA-HRP
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <1122317740.42e535ac936d3 <@t> mail.ucla.edu>
Content-Type: text/plain; charset=ISO-8859-1
We are about to cut a brain that was injected with
WGA-HRP for tract tracing. We haven't done this
for quite a while (decades?) and don't remember
if it is possible to delay processing after cutting.
If it is possible, how long can we delay, and what
solution should the cut tissue be stored in?
We plan to stain using the Olucha molybybdate
modification to the Mesulam TMB procedure.
Bob Nienhuis
UCLA / VA Medical Center
Los Angeles, CA
------------------------------
Message: 5
Date: Mon, 25 Jul 2005 15:07:50 -0500
From: "Walters, Katherine S" <katherine-walters <@t> uiowa.edu>
Subject: [Histonet] historesin
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
<A4AA05CE92DACC43886D133DB94A926E0E995F4D <@t> medicine-exch1.medicine.uiowa.
edu>
Content-Type: text/plain; charset="us-ascii"
I have a colleague looking to buy some historesin. Apparently it is
made by Leica (in Germany), and they will no longer ship it to the US.
Is there a company here that can sell it, or a similar product?
Thanks,
Kathy
------------------------------
Message: 6
Date: Mon, 25 Jul 2005 15:11:15 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: equivalent to Re: [Histonet] historesin
To: "Walters, Katherine S" <katherine-walters <@t> uiowa.edu>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050725145504.01b275e0 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
I don't recall plastic type of Historesin since it has been out of
circulation for a while in USA, but if this is GMA, buy Technovits 7100
If it is the methylmethacrylate, MMA then buy Technovits 9100.
Electron Microscopy Sciences sells both kits. These are excellent
products.
At 02:07 PM 7/25/2005, you wrote:
>I have a colleague looking to buy some historesin. Apparently it is
>made by Leica (in Germany), and they will no longer ship it to the US.
>Is there a company here that can sell it, or a similar product?
>
>Thanks,
>Kathy
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 7
Date: Mon, 25 Jul 2005 18:47:26 -0400
From: "Morken, Tim - Labvision" <tpmorken <@t> labvision.com>
Subject: [Histonet] Janella Seaton...please contact me
To: "'Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0556BE8AC5551E4E8AF6BB9E42509BA20328CF55 <@t> usca0082k08.labvision.apogent.
com>
Content-Type: text/plain
Janella, please contact me about the database you want.
From: Janella Seaton
------------------------------------------------------------------------
----
----
Hello!
We want to implement a database for specimens we receive in our lab.
We would use this database starting with logging in specimens and ending
with archiving of specimens. It would be used to track when various
steps
(gross photos, processing, staining, etc) have occurred and by whom.
We do want a system that is user friendly.
Any suggestions or advice?
Thanks for the help.....
j.s.
Tim Morken
------------------------------
Message: 8
Date: Tue, 26 Jul 2005 18:44:09 +1000
From: Cathy Malcontenti-Wilson <cmalc <@t> unimelb.edu.au>
Subject: [Histonet] Background staining: mouse on mouse Broad spectrum
kit
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.2.1.2.2.20050726182055.02ecbce8 <@t> mail.staff.unimelb.edu.au>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Dear All,
I really need some advice regarding an ongoing intense background
problem.
We use FFP sections of mouse liver.
We are using an immuno kit called Zymed Histomouse Max Kit (It is a HRP-
DAB one).
Our primary ab is mouse anti human VEGF (1:1000).
The kit is called a BROAD SPECTRUM kit because it is meant to be able to
be
used for mouse, rabbit, rat and guinea pig primaries.
The secondary antibody is not completely explained in the kit but I
think
it is of goat host species.
Question: If it is broad spectrum does this mean that the secondary
antibody is a mixture of different antibodies?
The kit contains its own non specific block, with no details of what it
actually is.
Question: If the secondary ab is raised in goat, is this non specific
block
normal goat serum? If not, what could it be?
We know that when I incubate the tissue with DAB only, we get endogenous
peroxidase staining which is pretty much abolished with H2O2 block.
We know that when we omit the primary antibody and keep all the other
layers, we get intense background staining of the sinusoidal
(endothelial)
lining of practically all the sinusoids, and we get brown staining in
plasma cells, macrophages, connective tissue and other cells within
vessels.
Question: Omission of non specific blocking reagent showed increased
background staining. Does this mean that the blocking agent is actually
blocking some of the background but not all? Why not?
We know that when we omit both the primary antibody and the non specific
block then we get background staining.
Question: What does this mean?
We know if we omit the secondary antibody only, we get no staining.
Question: What does this mean?
I would greatly appreciate any input into this problem which has been
plaguing us for a while now.
I hope someone can help, I cant beleive what a great wealth of knowledge
there is on this histonet list.
Cathy Malcontenti-Wilson
------------------------------
Message: 9
Date: Tue, 26 Jul 2005 07:32:06 -0500
From: Don.Birgerson <@t> leica-microsystems.com
Subject: Re: [Histonet] historesin
To: "Walters, Katherine S" <katherine-walters <@t> uiowa.edu>
Cc: histonet-bounces <@t> lists.utsouthwestern.edu,
histonet <@t> pathology.swmed.edu
Message-ID:
<OF3E1490E5.43943AC1-ON8625704A.0043C8DC-8625704A.0044E73B <@t> leica-microsy
stems.com>
Content-Type: text/plain; charset="US-ASCII"
Hi Kathy,
While we quit importing Historesin, there is product called
"Technovit " that is sold in the US by "Energy Beam Science" at
413-786-9322. If you have further questions, please call me.
Best regards,
Don
Don Birgerson
Leica Microsystems
Technical Assistance Center
Don.Birgerson <@t> Leica-Microsystems.Com
1-800-248-0123 ext 5918
"Walters, Katherine S" <katherine-walters <@t> uiowa.edu>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07/25/2005 03:07 PM
To
<histonet <@t> pathology.swmed.edu>
cc
Subject
[Histonet] historesin
I have a colleague looking to buy some historesin. Apparently it is
made by Leica (in Germany), and they will no longer ship it to the US.
Is there a company here that can sell it, or a similar product?
Thanks,
Kathy
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_____________________________________________________________________
This e-mail has been scanned for viruses by MCI's Internet Managed
Scanning
Services - powered by MessageLabs. For further information visit
http://www.mci.com
------------------------------
Message: 10
Date: Tue, 26 Jul 2005 07:43:00 -0500
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com>
Subject: Re: [Histonet] CD 31
To: Angela McNabola <angela.mcnabola.b <@t> bayer.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF5BFE2A12.1853D9CE-ON8625704A.0045BBFE <@t> northamerica.intra.abbott.com>
Content-Type: text/plain; charset="us-ascii"
Santa Cruz gave me the same story - it's amazing that they depend on one
goat for a product line.
Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist
Discovery Cancer R4N2 AP3
847-938-4919
Jackie.O'Connor <@t> abbott.com
Angela McNabola <angela.mcnabola.b <@t> bayer.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07/25/2005 05:40 AM
To: histonet <@t> lists.utsouthwestern.edu
cc:
Subject: Re: [Histonet] CD 31
Which antibody are you using? We have had great success with Santa Cruz
(goat
polyclonal) until I ordered a new lot this past month. When I called
them, they
indicated to me that they were having problems with their goat,
literally.
I
guess it had stopped producing antibodies to CD31 that work in IHC
(interestingly enough, they still work in Westerns). They hope to have
the
problem resolved within the next month, with a new goat.
Angela McNabola, MS, HT(ASCP)SLS, QIHC
Bayer Healthcare
400 Morgan Lane
West Haven, CT 06516
"Kim Allred"
<kallred <@t> bellsouth.net> To:
<histonet <@t> lists.utsouthwestern.edu>
Sent by: cc:
histonet-bounces <@t> lists.utsouth Subject:
[Histonet] CD 31
western.edu
07/22/2005 03:15 PM
I am having a problem getting my CD31 to work. The protocol was for an
overnight incubation and pepsin pretreatment. I couldn't get it to work
this way so I altered it a bit by using trilogy for AR and a two hour
incubation for the primary ab. Does anyone have any ideas? This seemed
to
work well for a while but once again, I'm having problems with it. Any
input would be appreciated. Thanks in advance!
Kim Allred
Dermatopathology Associates
Jackson, MS
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Tue, 26 Jul 2005 09:18:10 EDT
From: MajorFocus <@t> aol.com
Subject: [Histonet] North Carolina Histologist Position Available
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <b7.5c99a6d9.30179212 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Frye Regional Medical Center in Hickory, North Carolina is currently
seeking a Histology Technician or Technologist for full time
employment.
Qualified applicants must be certified by the ASCP or equivalent
agency.
Please
apply on-line at _www.fryemedctr.com_ (http://www.fryemedctr.com/) or
call
toll-free (866) 494-4747.
Frye Regional Medical Center
Human Resources
420 North Center Street
Hickory, NC 28601.
------------------------------
Message: 12
Date: Tue, 26 Jul 2005 09:25:08 -0400
From: Pamela Marcum <pmarcum <@t> vet.upenn.edu>
Subject: Re: equivalent to Re: [Histonet] historesin
To: Gayle Callis <gcallis <@t> montana.edu>, "Walters, Katherine S"
<katherine-walters <@t> uiowa.edu>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID: <6.1.1.1.2.20050726092213.019799b0 <@t> mail.vet.upenn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Hi,
The Technovit 8100 is the same as JB-4 Plus which has a second polymer
to
make the GMA remain clearer and somewhat less brittle. As Don was kind
enough to give you the number for EBS I shall not repeat and you can
reach
Polysciences at 800-523-2575.
Pam Marcum
At 05:11 PM 7/25/2005, Gayle Callis wrote:
>I don't recall plastic type of Historesin since it has been out of
>circulation for a while in USA, but if this is GMA, buy Technovits
7100
>
>If it is the methylmethacrylate, MMA then buy Technovits 9100.
>
>Electron Microscopy Sciences sells both kits. These are excellent
products.
>
>At 02:07 PM 7/25/2005, you wrote:
>
>
>>I have a colleague looking to buy some historesin. Apparently it is
>>made by Leica (in Germany), and they will no longer ship it to the US.
>>Is there a company here that can sell it, or a similar product?
>>
>>Thanks,
>>Kathy
>>
>>
>>_______________________________________________
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Tue, 26 Jul 2005 09:49:42 -0400
From: Luis Chiriboga <Luis.Chiriboga <@t> med.nyu.edu>
Subject: [Histonet] Looking 4 Antibodies
To: Histonet <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <KFEIIJOCLBABEKFDAEHPAEFEFCAA.Luis.Chiriboga <@t> med.nyu.edu>
Content-Type: text/plain; charset="iso-8859-1"
HI everyone
I'm looking for antibodies against MLH-1, MSH-2, BRCA-1 and BRCA-2 for
use
in human FF-PE tissue. recommendations as well as comments are welcome.
feel free to respond directly to my address.
Thanks in advance
Luis
------------------------------
Message: 14
Date: Tue, 26 Jul 2005 10:55:00 -0400
From: <pathrm35 <@t> adelphia.net>
Subject: [Histonet] DIF procedures
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<10787860.1122389700521.JavaMail.root <@t> web2.mail.adelphia.net>
Content-Type: text/plain; charset=utf-8
Fellow Techs,
Does anyone have Direct Immunofluorescence procedures they are willing
to
share? We are interested in CD3, fibrinogen, IgA, IgM and IgG.
Thanks in advance.
Ron Martin, BS HT (ASCP) HTL
fax 561-721-1249
------------------------------
Message: 15
Date: Tue, 26 Jul 2005 11:01:06 -0400
From: <pathrm35 <@t> adelphia.net>
Subject: [Histonet] teaching heads for Nikon microscope
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<28528193.1122390066380.JavaMail.root <@t> web2.mail.adelphia.net>
Content-Type: text/plain; charset=utf-8
Fellow Techs,
Does anyone know of or where I can find binocular teaching heads for a
(older
model) multiheaded Nikon Optiphot microscope? Any leads or info is
greatly
appreciated.
Thanks in advance.
Ron Martin, BS HT (ASCP) HTL
fax 561-721-1249
------------------------------
Message: 16
Date: Tue, 26 Jul 2005 08:35:52 -0700 (PDT)
From: Histology SLU <sluhisto <@t> yahoo.com>
Subject: [Histonet] Integrase Interactor 1 (INI-1/hsnf5) antibody
needed
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050726153552.54597.qmail <@t> web51005.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello All:
I have a doc looking for the above antibody for use in FFPE tissue
sections of
rhabdoid tumor in children. Any help would be greatly appreciated.
Thanks, in
advance.
Susan
__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com
------------------------------
Message: 17
Date: Tue, 26 Jul 2005 11:58:13 -0400
From: "Louro, Pedro" <pedro.louro <@t> spcorp.com>
Subject: [Histonet] looking for iNOS antibody
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<4508920F80C0D411B90200508BF9A9F4062B4A40 <@t> LAFMSG30.us.schp.com>
Content-Type: text/plain
Hi all,
I'm starting a new experiment with rat mesentery artery looking for
inducible nitric oxide synthase
(iNOS, eNOS, and nNOS).
What companies would carry this antibody that would work on FFPE rat
tissue.
Also, what would be a good positive control?
Thanks in advance for your assistance
Pedro
*********************************************************************
This message and any attachments are solely for the intended recipient.
If you
are not the intended recipient, disclosure, copying, use or distribution
of the
information included in this message is prohibited -- Please immediately
and
permanently delete.
------------------------------
Message: 18
Date: Tue, 26 Jul 2005 12:13:31 -0400
From: Dana Settembre <settembr <@t> umdnj.edu>
Subject: Re: [Histonet] Integrase Interactor 1 (INI-1/hsnf5) antibody
needed
To: histonet <@t> lists.utsouthwestern.edu, sluhisto <@t> yahoo.com
Message-ID: <s2e62902.026 <@t> smtpnpc.umdnj.edu>
Content-Type: text/plain; charset=US-ASCII
I purchased it from BD Biosciences from San Diego, CA cat# 612110
I pretreat with DakoCytomation's Target Retrieval Solution. and use it
at 1:50 incubating for 15 minutes room temp.
Works nicely but there's not much in the vial and I am looking to Santa
Cruz, who sells 1ml of it.
Santa Cruz Cat. sc-13055
Good Luck.
Dana Settembre
University Hospital-UMDNJ
Newark, NJ
>>> Histology SLU <sluhisto <@t> yahoo.com> 7/26/2005 11:35:52 AM >>>
Hello All:
I have a doc looking for the above antibody for use in FFPE tissue
sections of rhabdoid tumor in children. Any help would be greatly
appreciated. Thanks, in advance.
Susan
__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Tue, 26 Jul 2005 10:48:10 -0600
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: [Histonet] IgG2a and IgG1 for guinea pig
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000101c59201$ce33a1c0$a7d48a80 <@t> AMY>
Content-Type: text/plain; charset="US-ASCII"
Hello everyone
I have been looking for some reagents for guinea pig for both ELISA and
IHC for IgG2a and IgG1. I did find a source for the IgG2a through
Nordic Immuno Reagents. Has anyone had any dealings with them? They
have several antibodies against guinea pig IgG2a all derived from
different species (sheep, goat and rabbit). I was guessing that the
rabbit antibody would be nice since I could use a polymer detection
system if necessary. Any suggestions of a different vendor or anything
else would be appreciated.
Thanks in advance.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 20, Issue 35
****************************************
------------------------------
Message: 4
Date: Tue, 26 Jul 2005 14:41:25 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] Genta stain for Helicobacter pylori
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE0171758F <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="ISO-8859-1"
It's in the archives, here:
http://www.histosearch.com/histonet/Nov99/RE.GENTASTAINA.html
I read a paper which stated that lead nitrate can be substituted for
uranium
nitrate in this procedure, thereby avoiding the problems of handling and
disposing of radioactive material.
Paul M.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> IamNinaOwen <@t> aol.com
> Sent: Tuesday, July 26, 2005 11:29 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Genta stain for Helicobacter pylori
>
> Hello Histonetters!
>
> Has anyone ever used the Genta stain for Helicobacter pylori? I'm
trying
> to
> find a protocol for this method, so any help will be very much
> appreciated!
>
> Thanks
>
> Nina Owen
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 5
Date: Tue, 26 Jul 2005 14:43:45 -0400
From: Diana McCaig <dmccaig <@t> ckha.on.ca>
Subject: [Histonet] granular debris in paraffin blocks
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <3E5A3F039F0BD8118B4700C00D00202404361E <@t> CKHA9>
Content-Type: text/plain
Does anyone have any suggestions to obtain sections from an autopsy case
of
a drowning victim. The lung tissue has granular debris from a muddy
ditch.
The pathologist wants this demonstrated but it is virtually impossible
to
obtain anything due to fragmentation of the blocks from this.
Thanks
Diana
------------------------------
Message: 6
Date: Tue, 26 Jul 2005 14:48:38 -0400
From: "Kristopher Kalleberg" <Kristopher.Kalleberg <@t> unilever.com>
Subject: [Histonet] re: iNOS
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<ISSMTP.2002_3_.20050726144838.2528F <@t> usl-006.na.unilever.com>
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
If I remember right Neomarkers/Lab Vision Corp. has iNOS antibodies. I
used
them maybe 1 1/2 years ago but never got great results from the stain.
I ran
the test numerous times and was unable to solve the problem. If you
decide to
use Neomarkers and are able to achieve good results please send me your
secret.
Good luck.
Kris Kalleberg
Histologist
Unilever R&D
40 Merritt Blvd.
Trumbull, CT 06611
203 381 5765
------------------------------
Message: 7
Date: Tue, 26 Jul 2005 14:51:00 -0400
From: "Joanne Mauger" <MAUGER <@t> email.chop.edu>
Subject: Re: [Histonet] Integrase Interactor 1 (INI-1/hsnf5) antibody
needed
To: <histonet <@t> lists.utsouthwestern.edu>,<sluhisto <@t> yahoo.com>
Message-ID: <s2e64de5.006 <@t> email.chop.edu>
Content-Type: text/plain; charset=US-ASCII
Susan,
You can get INI-1(BAF47) from BD Transduction labs- catalog # 612110
for 50 ug, or 61211 for 150 ug.
Jo
>>> Histology SLU <sluhisto <@t> yahoo.com> 07/26/05 11:35 AM >>>
Hello All:
I have a doc looking for the above antibody for use in FFPE tissue
sections of rhabdoid tumor in children. Any help would be greatly
appreciated. Thanks, in advance.
Susan
__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Tue, 26 Jul 2005 14:53:05 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] Applied IHC Journal
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s2e64e75.002 <@t> hcnwgwds01.hh.chs>
Content-Type: text/plain; charset=US-ASCII
Does anyone have the Volume 3, Number 1 issue of Applied
Immunohistochemistry from 1995?
Thank you!
Richard
Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596
(860) 545-0174 Fax
-----------------------------------------------
Confidentiality Notice
This e-mail message, including any attachments, is for the sole use of
the intended recipient(s) and may contain confidential or proprietary
information which is legally privileged. Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are not the intended
recipient, please promptly contact the sender by reply e-mail and
destroy all copies of the original message.
------------------------------
Message: 9
Date: Tue, 26 Jul 2005 15:12:19 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] granular debris in paraffin blocks
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE01717590 <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="ISO-8859-1"
Now there's a unique problem! No, sand really doesn't section very well.
How
about cutting a thick section? A 20 or 30 micron section may not be
suitable for cellular detail, but it might contain enough of the
material to
demonstrate it adequately; and while the section might be full of
striations, hopefully the striations might not be deep enough to shread
the
ribbon. Also, try to get the earliest section you can get, while knife
damage is minimal. Use a fresh section of knife edge, and start rotating
the
microtome before the block touches the knife, so you can get the first
couple of sections off the block face. If that doesn't work, your
pathologist will probably have to demonstrate the debris by having the
lung
x-rayed.
Paul M.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
Diana
> McCaig
> Sent: Tuesday, July 26, 2005 11:43 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] granular debris in paraffin blocks
>
> Does anyone have any suggestions to obtain sections from an autopsy
case
> of
> a drowning victim. The lung tissue has granular debris from a muddy
> ditch.
> The pathologist wants this demonstrated but it is virtually impossible
to
> obtain anything due to fragmentation of the blocks from this.
> Thanks
> Diana
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 10
Date: Tue, 26 Jul 2005 12:19:51 -0700 (PDT)
From: GT Hebert <emerald_lake77 <@t> yahoo.com>
Subject: [Histonet] Serotec Rat Anti-mouse F4/80 : Biotin -- anyone
know of good concentration?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050726191951.92792.qmail <@t> web31708.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello once again,
I just received my Serotec Rat Anti-mouse F4/80 antibody and was
wondering if anyone can share with me the concentration they use --
closest to optimized would be great!
Thanks
Gustave Hebert
Scientist II
CVMD
Wyeth - Cambridge
__________________________________________________
Do You Yahoo!?
Tired of spam? Yahoo! Mail has the best spam protection around
http://mail.yahoo.com
------------------------------
Message: 11
Date: Tue, 26 Jul 2005 12:23:10 -0700 (PDT)
From: GT Hebert <emerald_lake77 <@t> yahoo.com>
Subject: [Histonet] Biotin conjugated Antibodies -- can we skip
biotin??
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050726192310.49951.qmail <@t> web31711.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hello,
Just a general question to help increase my knowledge:
If I had an antibody that was conjugated to biotin -- say for example, a
goat anti-mouse P-selectin (biotin) -- can I just take a rabbit
anti-goat conjugated to AP and run IHC that way -- hopefully avoiding
the ABC step and background OR does the biotin from the primary Ab block
secondaries from coming in?
Thanks.
---------------------------------
Yahoo! Mail
Stay connected, organized, and protected. Take the tour
------------------------------
Message: 12
Date: Tue, 26 Jul 2005 12:45:44 -0700
From: "Glenn Krasinski" <gkrasinski <@t> covx.com>
Subject: [Histonet] anti-mouse cleaved PARP for IHC...
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <6837C00A0CC6594197C2F0EEF256561805AADA <@t> covxmail.covx.com>
Content-Type: text/plain; charset="us-ascii"
I am looking for a mouse-specific anti-cleaved PARP antibody that works
in IHC. Cell Signaling has a rabbit polyclonal that work in IC and WB,
but not in IHC.
Many thanks.
Glenn M. Krasinski
Scientist, Biology
CovX Research LLC
9381 Judicial Drive, Suite 200
San Diego, CA 92121
Ph: (858) 964-2053
Fax: (858) 964-2090
E-dress: gkrasinski <@t> covx.com
------------------------------
Message: 13
Date: Tue, 26 Jul 2005 14:45:22 -0500
From: Amy Johnson <ajohnson <@t> aipathology.com>
Subject: [Histonet] CK7 control/ CK20 contol
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <016A64931E1ED511B12C0002B302608052334F <@t> SERV001>
Content-Type: text/plain; charset="iso-8859-1"
What do most of you out there use for a CK7 control?......CK20
control?....
We currently are using an adenocarcinoma of the lung for our CK7 and an
adenocarcinoma of the colon for the CK20. We have been having problems
and
need some help.
Thanks
Amy Johnson
------------------------------
Message: 14
Date: Tue, 26 Jul 2005 14:56:03 -0500
From: "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu>
Subject: RE: [Histonet] CK7 control/ CK20 contol
To: "Amy Johnson" <ajohnson <@t> aipathology.com>, "Histonet \(E-mail\)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D6B654003615874B873E15BA680E2D221199F279 <@t> uwhis-xchng1.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
We mainly use liver for CK7 (stains the bile ducts) and colon
adenocarcinoma for CK20.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy
Johnson
Sent: Tuesday, July 26, 2005 2:45 PM
To: Histonet (E-mail)
Subject: [Histonet] CK7 control/ CK20 contol
What do most of you out there use for a CK7 control?......CK20
control?.... We currently are using an adenocarcinoma of the lung for
our CK7 and an adenocarcinoma of the colon for the CK20. We have been
having problems and need some help. Thanks
Amy Johnson
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Tue, 26 Jul 2005 14:13:26 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Re: Serotec Rat Anti-mouse F4/80 : Biotin --
anyone know of good concentration?
To: GT Hebert <emerald_lake77 <@t> yahoo.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050726141256.01b7ea60 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Fro paraffin (FFPE) or frozen sections?
At 01:19 PM 7/26/2005, you wrote:
>Hello once again,
>
>I just received my Serotec Rat Anti-mouse F4/80 antibody and was
wondering
>if anyone can share with me the concentration they use -- closest to
>optimized would be great!
>
>Thanks
>
>Gustave Hebert
>Scientist II
>CVMD
>Wyeth - Cambridge
>
>
>__________________________________________________
>Do You Yahoo!?
>Tired of spam? Yahoo! Mail has the best spam protection around
>http://mail.yahoo.com
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 16
Date: Tue, 26 Jul 2005 13:15:46 -0700 (PDT)
From: GT Hebert <emerald_lake77 <@t> yahoo.com>
Subject: [Histonet] Re: Serotec Rat Anti-mouse F4/80 : Biotin --
anyone know of good concentration?
To: Gayle Callis <gcallis <@t> montana.edu>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050726201546.63399.qmail <@t> web31711.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Sorry Gayle,
This would be for FFPE.
Thanks.
Gustave
Gayle Callis <gcallis <@t> montana.edu> wrote:
Fro paraffin (FFPE) or frozen sections?
At 01:19 PM 7/26/2005, you wrote:
>Hello once again,
>
>I just received my Serotec Rat Anti-mouse F4/80 antibody and was
wondering
>if anyone can share with me the concentration they use -- closest to
>optimized would be great!
>
>Thanks
>
>Gustave Hebert
>Scientist II
>CVMD
>Wyeth - Cambridge
>
>
>__________________________________________________
>Do You Yahoo!?
>Tired of spam? Yahoo! Mail has the best spam protection around
>http://mail.yahoo.com
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
---------------------------------
Start your day with Yahoo! - make it your home page
------------------------------
Message: 17
Date: Tue, 26 Jul 2005 14:22:05 -0600
From: Gayle Callis <gcallis <@t> montana.edu>
Subject: [Histonet] Re: Biotin conjugated Antibodies -- can we skip
biotin??
To: GT Hebert <emerald_lake77 <@t> yahoo.com>,
Histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6.0.0.22.1.20050726141359.01b7ee00 <@t> gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
Yes, you do not need more biotin in the system. When you work with
biotinylated primaries, all you need to do is use Strepavidin-HRP or
Strepavidin-AP, followed by the appropriate chromogen.
You eliminate the secondary conjugated to biotin completely, and also
the
ABC complex, and but be sure to use a good reliable Strepavidin-HRP or
AP. Southern Biotechnology has these as does Biosource/TAGO. The
primary
conjugated to biotin provides all the biotin you will need.
One of the perks of working with biotinylated primaries - it shortens
the
protocol!
IF you use Vectors ABC complex, DON"T or you will experience loss of
sensitivity and have poor staining. You will still need to do the
appropriate blocking, i.e. avidin/biotion (OR Strepavidin/biotin block
from
Vector) normal serum, proper diluents with serums, etc. to avoid
background problems.
We do biotinylated primaries almost exclusively and with great success
for
murine CD markers IHC and IFA (using Molecular Probes Strepavidin-Alexa
fluorophore conjugates.) If you want to discuss this more, contact me
privately.
At 01:23 PM 7/26/2005, you wrote:
>Hello,
>
>Just a general question to help increase my knowledge:
>
>If I had an antibody that was conjugated to biotin -- say for example,
a
>goat anti-mouse P-selectin (biotin) -- can I just take a rabbit
anti-goat
>conjugated to AP and run IHC that way -- hopefully avoiding the ABC
step
>and background OR does the biotin from the primary Ab block secondaries
>from coming in?
>
>Thanks.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
------------------------------
Message: 18
Date: Tue, 26 Jul 2005 16:25:10 -0400
From: "Woodward, Denise" <denise.woodward <@t> uconn.edu>
Subject: [Histonet] Artifact pictures and cell block prep
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F08C21376038414F937A74D8BC98AC9EABC28C <@t> EXCHANGEA.mgmt.ad.uconn.edu>
Content-Type: text/plain; charset="US-ASCII"
Hi all,
I'm looking for pictures of sectioning, staining and processing
artifacts. Anyone know where I might be able to find some on the web?
Also, looking for a reference for making cell blocks from body fluids.
Anyone know of a good source I can provide my histo students?
Many thanks to you all,
Denise
Denise Long Woodward, MS, HT(ASCP), HTL, QIHC
Dept. of Pathobiology and Veterinary Sciences
University of Connecticut
------------------------------
Message: 19
Date: Tue, 26 Jul 2005 16:31:09 EDT
From: Dndsomi <@t> aol.com
Subject: [Histonet] Productivity
To: histonet <@t> pathology.swmed.edu
Message-ID: <82.2cd8fb46.3017f78d <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
We've just been asked to start turning in our histology procedures to be
included in our labs daily productivity. How should we report our
workload; #
of cassettes, blocks, slides, special stains ? Any information will
surely be
appreciated.
Thanks,
DDietz, HT
------------------------------
Message: 20
Date: Tue, 26 Jul 2005 13:34:36 -0700
From: Christian Franci <cfranci <@t> rigel.com>
Subject: [Histonet] bunny Ab's
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5f251aaa45b280ca9b47daed7b9455c6 <@t> rigel.com>
Content-Type: text/plain; charset=US-ASCII; format=flowed
Hello Kids!
Was just wondering if any of you have a good supplier of rabbit anti
rat Ab's that are mouse absorbed (for real). Supposedly I purchased
one but, sans primary antibody present, it makes my mouse tissue light
up like a xmas tree.... talk about false positive!
thanks in advance.
Chris
------------------------------
Message: 21
Date: Tue, 26 Jul 2005 16:34:48 -0400
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] Artifact pictures and cell block prep
To: "Woodward, Denise" <denise.woodward <@t> uconn.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <42E69E68.1030302 <@t> umdnj.edu>
Content-Type: text/plain; format=flowed; charset=us-ascii
The AFIP manual (3rd edition, 1968, I don't know about other editions)
has photos of artifacts after the last numbered chapter.
For electron microscopy, there is a book called "An Atlas of Artifacts",
I don't remember the author off the top of my head.
I can't offer any suggestions for your body fluid question.
Geoff
Woodward, Denise wrote:
>Hi all,
>
>I'm looking for pictures of sectioning, staining and processing
>artifacts. Anyone know where I might be able to find some on the web?
>
>
>Also, looking for a reference for making cell blocks from body fluids.
>Anyone know of a good source I can provide my histo students?
>
>Many thanks to you all,
>Denise
>
>Denise Long Woodward, MS, HT(ASCP), HTL, QIHC
>Dept. of Pathobiology and Veterinary Sciences
>University of Connecticut
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 22
Date: Tue, 26 Jul 2005 16:40:58 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] Artifact pictures and cell block prep
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<09C945920A6B654199F7A58A1D7D1FDE01717591 <@t> lsexch.lsmaster.lifespan.org>
Content-Type: text/plain; charset="ISO-8859-1"
You might be interested in one of the following, if you can find a copy:
Atlas of Microscopic Artifacts and Foreign Materials by Yeh, Brooks &
Pietra
An Atlas of Artifacts Encountered in the Preparation of Microscopic
Tissue
Sections by Thompson & Luna
You might find them through www.bookfinder.com
We do a fair amount of cell block work in our lab, and have several
protocols for different kinds of fluids and cell suspensions. Is there
anything in particular you are looking for?
Paul M.
> ----------
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Woodward, Denise
> Sent: Tuesday, July 26, 2005 1:25 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Artifact pictures and cell block prep
>
> Hi all,
>
> I'm looking for pictures of sectioning, staining and processing
> artifacts. Anyone know where I might be able to find some on the web?
>
>
> Also, looking for a reference for making cell blocks from body fluids.
> Anyone know of a good source I can provide my histo students?
>
> Many thanks to you all,
> Denise
>
> Denise Long Woodward, MS, HT(ASCP), HTL, QIHC
> Dept. of Pathobiology and Veterinary Sciences
> University of Connecticut
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 23
Date: Tue, 26 Jul 2005 16:52:16 -0400
From: "Woodward, Denise" <denise.woodward <@t> uconn.edu>
Subject: RE: [Histonet] Artifact pictures and cell block prep
To: "Geoff McAuliffe" <mcauliff <@t> umdnj.edu>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<F08C21376038414F937A74D8BC98AC9EAB0022 <@t> EXCHANGEA.mgmt.ad.uconn.edu>
Content-Type: text/plain; charset="US-ASCII"
Sorry, I didn't specify, but I'm looking for artifact pictures ON-LINE,
so my students can access them from home.
Thanks,
DLW
Denise Long Woodward, MS, HT(ASCP), HTL, QIHC
Dept. of Pathobiology and Veterinary Sciences
University of Connecticut
-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu]
Sent: Tuesday, July 26, 2005 4:35 PM
To: Woodward, Denise
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Artifact pictures and cell block prep
The AFIP manual (3rd edition, 1968, I don't know about other editions)
has photos of artifacts after the last numbered chapter.
For electron microscopy, there is a book called "An Atlas of Artifacts",
I don't remember the author off the top of my head.
I can't offer any suggestions for your body fluid question.
Geoff
Woodward, Denise wrote:
>Hi all,
>
>I'm looking for pictures of sectioning, staining and processing
>artifacts. Anyone know where I might be able to find some on the web?
>
>
>Also, looking for a reference for making cell blocks from body fluids.
>Anyone know of a good source I can provide my histo students?
>
>Many thanks to you all,
>Denise
>
>Denise Long Woodward, MS, HT(ASCP), HTL, QIHC
>Dept. of Pathobiology and Veterinary Sciences
>University of Connecticut
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
--
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
**********************************************
------------------------------
Message: 24
Date: Tue, 26 Jul 2005 14:05:17 -0700
From: "Linke_Noelle" <Linke_Noelle <@t> Allergan.com>
Subject: RE: [Histonet] bunny Ab's
To: "Christian Franci" <cfranci <@t> rigel.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D475C767BF2F6D438FD30E3984FCBB11BD5A9E <@t> irmail121.irvine.allergan.com>
Content-Type: text/plain; charset="us-ascii"
Hi Chris,
Try Jackson, they usually have everything in the barnyard!
Noelle Linke, BS, HTL(ASCP)QIHC
Allergan, Inc
2525 Dupont Drive RD-2A
Irvine, CA 92612
714-246-5568
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Christian Franci
Sent: Tuesday, July 26, 2005 1:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] bunny Ab's
Hello Kids!
Was just wondering if any of you have a good supplier of rabbit anti
rat Ab's that are mouse absorbed (for real). Supposedly I purchased
one but, sans primary antibody present, it makes my mouse tissue light
up like a xmas tree.... talk about false positive!
thanks in advance.
Chris
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 25
Date: Tue, 26 Jul 2005 16:46:40 -0500
From: "Henry, Charlene" <Charlene.Henry <@t> STJUDE.ORG>
Subject: [Histonet] un-subscribe
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5CB39BCA5724F349BCB748675C6CA1A2099A199A <@t> SJMEMXMB02.stjude.sjcrh.local>
Content-Type: text/plain; charset="US-ASCII"
Charlene Henry HT (ASCP), QIHC
Histology/Immunohistochemistry Section Head
Department of Pathology
St. Jude Children's Research Hospital
901-495-3191
fax 901-495-3100
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 20, Issue 36
****************************************
Privileged/Confidential information may be contained in this message.
The information contained in this message is intended only for the use
of the recipient(s) named above and their co-workers who are working on
the same matter. The recipient of this information is prohibited from
disclosing the information to any other party unless this disclosure has
been authorized in advance.
If you are not intended recipient of this message or any agent
responsible for delivery of the message to the intended recipient, you
are hereby notified that any disclosure, copying, distribution or action
taken in reliance on the contents of this message is strictly
prohibited. You should immediately destroy this message and kindly
notify the sender by reply E-Mail. Please advise immediately if you or
your employer does not consent to Internet E-Mail for messages of this
kind. Opinions, conclusions and other information in this message that
do not relate to the official business of the firm shall be understood
as neither given nor endorsed by it.
Privileged/Confidential information may be contained in this message. The information contained in this message is intended only for the use of the recipient(s) named above and their co-workers who are working on the same matter. The recipient of this information is prohibited from disclosing the information to any other party unless this disclosure has been authorized in advance.
If you are not intended recipient of this message or any agent responsible for delivery of the message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of this message is strictly prohibited. You should immediately destroy this message and kindly notify the sender by reply E-Mail. Please advise immediately if you or your employer does not consent to Internet E-Mail for messages of this kind. Opinions, conclusions and other information in this message that do not relate to the official business of the firm shall be understood as neither given nor endorsed by it.
More information about the Histonet
mailing list