[Histonet] Biotin conjugated Antibodies -- can we skip biotin??

[GT Hebert]

Hello,
 
Just a general question to help increase my knowledge:
 
If I had an antibody that was conjugated to biotin -- say for example, a goat anti-mouse P-selectin (biotin) -- can I just take a rabbit anti-goat conjugated to AP and run IHC that way -- hopefully avoiding the ABC step and background OR does the biotin from the primary Ab block secondaries from coming in?
 
Thanks.



		
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