[Histonet] Biotin conjugated Antibodies -- can we skip biotin??

GT Hebert emerald_lake77 <@t> yahoo.com
Tue Jul 26 14:23:10 CDT 2005

Just a general question to help increase my knowledge:
If I had an antibody that was conjugated to biotin -- say for example, a goat anti-mouse P-selectin (biotin) -- can I just take a rabbit anti-goat conjugated to AP and run IHC that way -- hopefully avoiding the ABC step and background OR does the biotin from the primary Ab block secondaries from coming in?

Yahoo! Mail
 Stay connected, organized, and protected. Take the tour

More information about the Histonet mailing list