[Histonet] stain for pre-collagen and mature collagen

Steven Coakley sjchtascp <@t> yahoo.com
Tue Jul 26 13:30:39 CDT 2005

I've been working on Herovica's Method for differentiating pre-collagen(young) verses mature collagen using varies combinations of methy blue and van gieson's.  Luna has a modified method on pg 453 of his "Histopathologic Methods...."  The regular Herovica stain calls for tissue fixed in formol-acetic-alcohol.  10% NBF leaves the muscle a blue to blue-green and very little red (mature collagen).  I tried post fixation in the FAA for 1 hr w/o allot of difference.
Bouins post-fixation seems to work best.  Certainly not getting the results as outlined.  Is there anyway to check the saturated picric acid?  Any ideas or suggestion would be appreciated, especially from those familiar with this stain.

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