[Histonet] stain for pre-collagen and mature collagen

[Steven Coakley]

I've been working on Herovica's Method for differentiating pre-collagen(young) verses mature collagen using varies combinations of methy blue and van gieson's.  Luna has a modified method on pg 453 of his "Histopathologic Methods...."  The regular Herovica stain calls for tissue fixed in formol-acetic-alcohol.  10% NBF leaves the muscle a blue to blue-green and very little red (mature collagen).  I tried post fixation in the FAA for 1 hr w/o allot of difference.
Bouins post-fixation seems to work best.  Certainly not getting the results as outlined.  Is there anyway to check the saturated picric acid?  Any ideas or suggestion would be appreciated, especially from those familiar with this stain.
 
Steve
 



		
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