[Histonet] CD 31

Jackie M O'Connor Jackie.O'Connor <@t> abbott.com
Tue Jul 26 07:43:00 CDT 2005

Santa Cruz gave me the same story - it's amazing that they depend on one 
goat for a product line. 

Jacqueline M. O'Connor HT(ASCP) QIHC
Assistant Scientist 
Discovery Cancer R4N2 AP3
Jackie.O'Connor <@t> abbott.com

Angela McNabola <angela.mcnabola.b <@t> bayer.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07/25/2005 05:40 AM

        To:     histonet <@t> lists.utsouthwestern.edu
        Subject:        Re: [Histonet] CD 31

Which antibody are you using?  We have had great success with Santa Cruz 
polyclonal) until I ordered a new lot this past month.  When I called 
them, they
indicated to me that they were having problems with their goat, literally. 
guess it had stopped producing antibodies to CD31 that work in IHC
(interestingly enough, they still work in Westerns).  They hope to have 
problem resolved within the next month, with a new goat.

Angela McNabola, MS, HT(ASCP)SLS, QIHC
Bayer Healthcare
400 Morgan Lane
West Haven, CT 06516

                      "Kim Allred"   
                      <kallred <@t> bellsouth.net>               To: 
<histonet <@t> lists.utsouthwestern.edu> 
                      Sent by:                              cc:            
                      histonet-bounces <@t> lists.utsouth        Subject: 
[Histonet] CD 31 
                      07/22/2005 03:15 PM    

I am having a problem getting my CD31 to work.  The protocol was for an
overnight incubation and pepsin pretreatment.  I couldn't get it to work
this way so I altered it a bit by using trilogy for AR and a two hour
incubation for the primary ab.  Does anyone have any ideas?  This seemed 
work well for a while but once again, I'm having problems with it.  Any
input would be appreciated.  Thanks in advance!

Kim Allred

Dermatopathology Associates

Jackson, MS

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