[Histonet] TMB staining for WGA-HRP

NIENHUIS,ROBERT nienhuis <@t> ucla.edu
Mon Jul 25 13:55:40 CDT 2005

We are about to cut a brain that was injected with
WGA-HRP for tract tracing. We haven't done this
for quite a while (decades?) and don't remember
if it is possible to delay processing after cutting.
If it is possible, how long can we delay, and what
solution should the cut tissue be stored in?
We plan to stain using the Olucha molybybdate
modification to the Mesulam TMB procedure.

Bob Nienhuis
UCLA / VA Medical Center
Los Angeles, CA

More information about the Histonet mailing list