gluteraldehyde fixation with Re: [Histonet] Rat brains
gcallis <@t> montana.edu
Mon Jul 25 12:21:28 CDT 2005
Question: is he planning to do EM on these tissues? If he had used
paraformaldehyde, your sectioning would be much easier - but if he is also
doing EM - you can try some things to counteract the dry/brittle brain.
In general, gluteraldehyde creates some friable, brittle tissues when
these are processed and embedded in paraffin. Check to see if you are
processing too long as long dehydrations, etc and heat from paraffin
contributes to the problem.
A good long soak in ice water or solid ice block with water on top after
trimming might help but be sure to not retrim the soak away, you have to
get the first sections off the knife. A warm water or room temperature
soak the going onto ice water for a time may help too, brain sometimes
needs a bit of help.
At 01:01 PM 7/23/2005, you wrote:
>A research project for our neuropathologist includes rat brains. These
>are extremely difficult to section as he profuses the whole rat with
>glutaraldehyde then takes brain samples. I have tried adding alcohol to
>my water bath but these sections still have many, many wrinkles. Can
>anyone offer suggestions? I would be so grateful for any and all advice.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)
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