[Histonet] (no subject)

[Dana Marshall]

hi,
we generally use less than that volume for rinse, approx 0.8 ml with gentle 
2-3 time up and down.  remove that 0.8 ml and repeat the process two more 
times, each time with 0.8ml..  the yield is good but it must be done very 
gently.  we then did a quick rbc lysis step.
hope this helps.
dana marshall
----- Original Message ----- 
From: "bcgirish" <bcgpath <@t> rediffmail.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, July 25, 2005 8:06 AM
Subject: [Histonet] (no subject)



Dear friends,

Does anyone do bronchoalveolar lavage in mice?
we anaesthetize the animals with pentobarbitone.With an incision at the 
lower cervical region,trachea will be exposed,fluid will be collected after 
canulation and injecting 2ml normal saline.This method is yielding lot of 
RBCs which interferes with the interpretation.We tried with even 
exsanguination of the animals with renal artery severing but the result was 
not that much encouraging.can anyone help me in this regard by a better 
method?





REGARDS
Dr.Girish B.Chandrashekar
Dept of Preclinical Safety Evaluation
Dr Reddys Lab
Hyderabad-49

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