[Histonet] Re: endogenous peroxidase blocking

Malam Jacqueline Jacqueline.Malam <@t> rli.mbht.nhs.uk
Mon Jul 25 02:39:50 CDT 2005


If you are performing IHC on really bloody specimens, you will be better off
using an alkaline phosphatase method rather than HRP-based - it will give
better results and be a lot less trouble. You may find you will affect the
staining of your antigen if you increase the H2O2.
Cheers
Jacqui Malam
Lancaster



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