[Histonet] under decal

[Bonner, Janet]

OPTION 1:  "redecal"  Melt the paraffin block, put the tissue in a cassette
as you would a fresh piece of tissue to process.  Drop it in xylene for 15
minutes x2, then 100% abs. alcohol x2, 95% abs. alcohol.  Wash in water and
drop back into the decal.  Process when soft.

OPTION 2:   "surface decal"  Pour some decal solution into a small container
or Petri dish and put the faced block face-down in the solution.  Let soak
for 15 min (time depends on hardness of the tissue) put on ice and try to
section. Only the surface will be decaled so try to take a section off the
surface.


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Trisha
Emry
Sent: Friday, July 22, 2005 5:44 PM
To: histo
Subject: [Histonet] under decal


Is there anything that can be done for under decalcified bone after it has
been processed?

Trisha
Seattle


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