[Histonet] cryosectioning liver/sucrose

Monfils, Paul PMonfils <@t> Lifespan.org
Fri Jul 22 15:17:52 CDT 2005

At any given temperature, tissues infiltrated with sucrose tend to be softer
and less completely frozen than similar tissues without sucrose (and 30% is
a very high level of sucrose), and therefore they compress and wrinkle more.
Try cutting them at a colder temperature than you would normally use for
liver.  That will probably help produce flatter sections with less

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> Steven Coakley
> Sent: 	Friday, July 22, 2005 12:27 PM
> To: 	Histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] cryosectioning liver/sucrose
> Something a "tad" new for me here.  I've never sectioned 4%Para liver
> infiltrated with 30% sucrose.  They sectioned well but the slide appear to
> be loaded with what appears to be folds throughout.  Not what I routinely
> see on regular unfixed FS.  Is this normal for fixed sucrose infiltrated
> sections.  Are there any special techniques when dealing with this special
> tissue.
> Steve
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