[Histonet] Black paraffin and cassettes

[Pamela Marcum]

At 10:10 AM 7/22/2005, Monfils, Paul wrote:
>I believe the dye Sudan Black B is soluble in paraffin.
>
>Black cassettes?  Nope.
>
> > ----------
> > From:         histonet-bounces <@t> lists.utsouthwestern.edu on behalf of
> > Steven P Postl
> > Sent:         Thursday, July 21, 2005 5:40 PM
> > To:   histonet <@t> lists.utsouthwestern.edu
> > Subject:      [Histonet] Black paraffin and cassettes
> >
> > A researcher is wondering (now so am I [I never heard this request
> > before]) if any company sells black paraffin and black embedding
> > cassettes?  No, this isn't a Gothic thing, a legitimate request.  Would it
> >
> > be possible to add something to paraffin to make it stay a black color and
> >
> > remain strong as our commercial paraffin products?  Thanks.
> >
> >
> >
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It is soluble in paraffin however it will be removed by reagents used in 
deparaffinization and staining.  Due to the additional materials added to 
raw paraffin it will be spotty.  It will also leave some precipitate in the 
tissue.

Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum <@t> vet.upenn.edu