[Histonet] Murine Blood Smear / Smears IHC
Favara, Cynthia (NIH/NIAID)
cfavara <@t> niaid.nih.gov
Thu Jul 21 19:15:57 CDT 2005
Questions - why are you dong this? What is the ultimate goal? If you just
want a differential then do a differential. If you are looking for various
populations of cells then FACS might be more reasonable and far simpler.
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From: GT Hebert [mailto:emerald_lake77 <@t> yahoo.com]
Sent: Thursday, July 21, 2005 3:30 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Murine Blood Smear / Smears IHC
I've searched but my answers so far have come up too vague.
I need to know if it is possible to do IHC on mouse blood smears???? If so,
how would one go about preparing and fixing the sample? Is there anything
I should watch out for?
I just want to run normal IHC (probably to mark certain white blood cells
(for example, monocytes to start).
Any assistance is valued and greatly appreciated. Thanks!
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