[Histonet] Murine Blood Smear / Smears IHC

Favara, Cynthia (NIH/NIAID) cfavara <@t> niaid.nih.gov
Thu Jul 21 19:15:57 CDT 2005

Questions - why are you dong this? What is the ultimate goal? If you just
want a differential then do a differential. If you are looking for various
populations of cells then FACS might be more reasonable and far simpler.

Cynthia Favara
903 South 4th Street
Hamilton, MT 59840

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-----Original Message-----
From: GT Hebert [mailto:emerald_lake77 <@t> yahoo.com] 
Sent: Thursday, July 21, 2005 3:30 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Murine Blood Smear / Smears IHC

Hello everyone,
I've searched but my answers so far have come up too vague.  
I need to know if it is possible to do IHC on mouse blood smears????  If so,
how would one go about preparing and fixing the sample?   Is there anything
I should watch out for?
I just want to run normal IHC (probably to mark certain white blood cells
(for example, monocytes to start). 
Any assistance is valued and greatly appreciated.  Thanks!
Gustave Hebert
Scientist II
Wyeth Research
Cambridge MA

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