[Histonet] PAP Staining

Rogerson Kemlo (ELHT) Pathology Kemlo.Rogerson <@t> elht.nhs.uk
Thu Jul 21 03:04:38 CDT 2005

Not with your question Jennifer; EA is a mix of eosin, light green and
Bismarck Green. If you run out of EA you can make it up from the
constituent dyes and chemicals. However the mix of dyes and chemicals
depend on the type of EA you require. EA65, EA50 whatever depends on
altering the amounts of dye and mordents. Classically EA50 is for Gynae
whilst EA65 is for Non-Gynae; the latter is 'pinker'. If you want to
make it up then tell me what you want it for or what your staining
preference is and I'll send you the recipe.


-----Original Message-----
From: Jennifer MacDonald [mailto:JMacDonald <@t> mtsac.edu] 
Sent: 20 July 2005 19:31
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PAP Staining

 If EA was not available, what would be a suitable replacement for EA in

the PAP stain,  eosin or light green?
Thank you.

Jennifer MacDonald
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