[Histonet] IHC with neomarker Rabbit anti Ki67 on paraffinbrainsections?

[Patsy Ruegg]

I have used rab. Mono ki67 from Lab Vision on several species with really
good results also with HIER with citrate ph6 buffer and rab. Labelled
polymer detection system.
Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gudrun Lang
Sent: Wednesday, July 20, 2005 2:55 PM
To: Histonetliste (Histonetliste)
Subject: AW: [Histonet] IHC with neomarker Rabbit anti Ki67 on
paraffinbrainsections?

Hi,
we tested this antibody on mamma-tissue. The result was very good. We will
use it in the future.
Benchmark XT, iview DAB, 1:100, 16 min; retrieval with EDTA

Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Yun Li
Gesendet: Dienstag, 19. Juli 2005 22:19
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] IHC with neomarker Rabbit anti Ki67 on paraffin
brainsections?

Greeting all,

I am trying to look at adult neurogenesis in the brain and would like to do
IHC with proliferation markers such as Ki67 and PCNA.  I came across this
new monoclonal rabbit anti-ki67 antibody and would like to know how well it
works, particularly on paraffin embedded tissues.  If anyone has used it and
wouldn't mind sharing with me condition and result, please drop a line.  I
appreciate it!!

yun
 


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