[Histonet] One Step Trichrome.

[Monfils, Paul]

I use the Gomori technique as my standard trichrome stain.  It's a "one-step
procedure" in that the collagen stain (light green FCF in the original
procedure, though I prefer to substitute aniline blue) and the plasma stain
(chromotrope 2R) are combined in one solution with phosphotungstic acid and
acetic acid, and the stain is progressive (no differentiation required).
Staining time is 20 minutes, though good results can be obtained in half
that time. The solution keeps well, at least six months at room temperature,
at least a year refrigerated.  If you need the protocol I can send it to
you.

Paul M.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Ian
> Montgomery
> Sent: 	Wednesday, July 20, 2005 9:11 AM
> To: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	[Histonet] One Step Trichrome.
> 
>          I'm considering using a one step trichrome for a zoology 
> undergraduate teaching class. As the material will come from invertebrates
> 
> is there a one step technique which would offer the best and most 
> consistent result.
> 
> Dr. Ian Montgomery,
> Histotechnology,
> Graham Kerr Building,
> Institute of Biomedical & Life Sciences,
> University of Glasgow,
> Glasgow,
> G12 8QQ.
> Tel: 0141 339 8855
> Office: 4652
> Lab: 6644.
> Pager: 07623 975451
> e-mail: ian.montgomery <@t> bio.gla.ac.uk  
> 
> 
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