[Histonet] transparent tissue

[ze'ev silverman]

We are trying to embed 300 micron thick, mouse olfactory bulb explants. 
The explants are about 3 mm diameter and become transparent following 
exposure to xylene, making it very difficult to orient them. Does 
anyone know how we might lightly stain or label the tissue, perhaps by 
some particulate material? Different stains and materials we have tried 
have not survived the ethanol or xylene steps.

Thanks,  ZS


Ze'ev Silverman, Ph.D.
Zlotowski Center for Neuroscience
Dept. of Morphology
Faculty of Health Sciences
P.O. Box 653
Beer Sheva 84105
Israel

972 8 647-7307 (office)
         8 647-7304 (lab)
         8 647-7627 (fax)
http://medic.bgu.ac.il/brain