[Histonet] mixing up primary and secondary antibodies for double/triple staining

Yves Heremans herme013 <@t> umn.edu
Tue Jul 19 13:14:08 CDT 2005

Dear Histonetters,
I have a general question on double or triple fluorescence stainings. 
Jackson Immunoresearch recommends doing sequential stainings, i.e. 
complete staining for antigen A first, then antigen B and then antigen 
C. Is there any danger in mixing up all the primary antibodies in one 
mix instead of using the sequential approach ? And, if using secondary 
antibodies derived from the same species, is there any danger in doing 
the same for the secondaries ?

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