[Histonet] PGP9.5
Dodson, Cecelia
CDodson <@t> clarian.org
Mon Jul 18 15:26:05 CDT 2005
We are staining PGP9.5 on a regular bases using a polyclonal antibody we purchase from Biogenesis. It is antigen retrieved in a steamer for 15 minutes then stained with LSAB2 from Dako. Cecelia
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, July 18, 2005 12:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 21
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. RE: dissecting board (Malam Jacqueline)
2. Need help with PGP9.5 on FFPE skin (Nicola Cragg)
3. RE: automated microtomes (Bonner, Janet)
4. Re: Dissection boards (Fred Underwood)
5. Bodies in morgue - who does the checking? (Paula Wilder)
----------------------------------------------------------------------
Message: 1
Date: Mon, 18 Jul 2005 12:39:23 +0100
From: Malam Jacqueline <Jacqueline.Malam <@t> rli.mbht.nhs.uk>
Subject: [Histonet] RE: dissecting board
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <B75B29D97DE3E84ABC2276497D44C33B0815FF7B <@t> rlixch>
Content-Type: text/plain
Thermo Shandon do 2 colours and 2 sizes of polyethylene board.
They're easy to clean and last. We actually got ours from a butchers'
suppliers!
-----Original Message-----
From: histonet-request <@t> lists.utsouthwestern.edu
[mailto:histonet-request <@t> lists.utsouthwestern.edu]
Sent: 17 July 2005 18:07
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 19
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than
"Re: Contents of Histonet digest..."
Today's Topics:
1. Dissection boards (Katia Cristina Catunda)
----------------------------------------------------------------------
Message: 1
Date: Sat, 16 Jul 2005 22:20:15 -0300
From: "Katia Cristina Catunda" <kccatunda <@t> terra.com.br>
Subject: [Histonet] Dissection boards
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <019801c58a6d$b0bbc050$a279fea9 <@t> privatexx>
Content-Type: text/plain; charset="iso-8859-1"
In our lab we still use wood-dissecting boards but we really want to change
it!! (wood can be very very very dirty after some years even if we submit
the boards to an intensive descontaminating process).
Would like some tips about what kind of material we should use it and what
is the best option, buy it from distributors like Mopec or to pay for
someone to make them?
Some simple questions that makes a lot of difference for us...
Thanks
Katia
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 20, Issue 19
****************************************
DISCLAIMER: This e-mail is confidential and privileged. If you are not the
intended recipient please accept our apologies; please do not disclose, copy
or distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful. Please
inform postmaster <@t> rli.mbht.nhs.uk that this message has gone astray before
deleting it. Comments or opinions expressed in this email are those of
their respective contributors only. The views expressed do not represent the
views of the Trust, its management or employees. Morecambe Bay Hospitals NHS
Trust is not responsible and disclaims any and all liability for the content
of comments written within.Thank you for your co-operation.
------------------------------
Message: 2
Date: Mon, 18 Jul 2005 13:39:29 +0100
From: "Nicola Cragg" <n.cragg <@t> epistem.co.uk>
Subject: [Histonet] Need help with PGP9.5 on FFPE skin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DFDB9D8E7F453A4D9C29C66DE3410D830864D4 <@t> server.epistem.local>
Content-Type: text/plain; charset="US-ASCII"
Hello,
I'm posting this message with some embarrassment and apologise for raising anybody's hope of a quck solution........
I have been trying to optimise PGP9.5 on FFPE skin samples (3 micron), using a mouse monoclonal (clone 10A1) from Neuromics. Despite my initial excitement expressed in an earlier posting, I have since found some very good photos of PGP9.5 staining in published work which has put mine to shame and has made me realise that my IHC is not working well enough at all. I've found an improvement with the waterbath antigen retrieval, as the dermis remains intact and there is some specific staining (I think) in the reticular dermis, which wasn't apparent with microwave antigen retrieval.
However, it appears that there seems to be more of a trend of using free-floating 50 micron sections. Is that because routine FFPE sections are difficult to stain for this antigen? Has anyone got it to work on FFPE samples? I was quite hopeful that it would work from reading the datasheet and I have also found that Dako make a Rabbit polyclonal for FFPE sections and they're usually excellent antibodies so I'm hoping to try. Any advice or suggestions will be gratefully received.
Regards,
Nicola Cragg
------------------------------
Message: 3
Date: Mon, 18 Jul 2005 08:43:37 -0400
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] automated microtomes
To: "'Sennello, Gina '" <gsennello <@t> osip.com>,
"'histonet-bounces <@t> lists.utsouthwestern.edu '"
<histonet-bounces <@t> lists.utsouthwestern.edu>, "'Histonet Histonet
(E-mail) '" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <07AB60D5D7B9754EBF56F360F98D083DEB4325 <@t> fh2k093.fhmis.net>
Content-Type: text/plain; charset=iso-8859-1
We have used the Leica's with great success and few if any call backs after
more than eight years!! Janet
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
To: Histonet Histonet (E-mail)
Sent: 7/13/2005 3:12 PM
Subject: [Histonet] automated microtomes
I am in the market for fully automated microtome and have looked at
Leica's RM2255, Thermo-Electron's Finesse ME and Microm's 355S. Does
anyone
have any strong feels for against any of these instruments?
Thanks in advance for you opinions and help.
Gina
Gina Sennello
Senior Associate Scientist
Histotechnologist
OSIP
2860 Wilderness Place
Boulder, CO
80301
phone 303-546-7739
fax 303-444-0672
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Mon, 18 Jul 2005 12:29:54 -0400
From: "Fred Underwood" <funderwood <@t> mcohio.org>
Subject: Re: [Histonet] Dissection boards
To: <histonet <@t> lists.utsouthwestern.edu>, <kccatunda <@t> terra.com.br>
Message-ID: <s2dba0c3.071 <@t> mcohio.org>
Content-Type: text/plain; charset=US-ASCII
I've found that kitchen cutting boards from your local department store
works well, and it's cheaper than from a medical supplier.
Fred
>>> "Katia Cristina Catunda" <kccatunda <@t> terra.com.br> 07/16/05 09:20PM
>>>
In our lab we still use wood-dissecting boards but we really want to
change it!! (wood can be very very very dirty after some years even if
we submit the boards to an intensive descontaminating process).
Would like some tips about what kind of material we should use it and
what is the best option, buy it from distributors like Mopec or to pay
for someone to make them?
Some simple questions that makes a lot of difference for us...
Thanks
Katia
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Mon, 18 Jul 2005 16:37:34 +0000
From: "Paula Wilder" <histo20 <@t> hotmail.com>
Subject: [Histonet] Bodies in morgue - who does the checking?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY102-F361D07094303B17CCBC79BA4D50 <@t> phx.gbl>
Content-Type: text/plain; format=flowed
Hi everyone!
Any feedback on whose responsibility it is to check bodies in the morgue at
your respective instituition would be greatly appreciated. So far, by
phoning neighboring hospitals, I have found that Security, a diener service,
or the Pathology Assistants are the ones responsible. Any help in this
would truly be greatly appreciated! Thanks so much!
Paula Wilder
St.Joseph Medical Center
Towson, MD 21204
410-337-1741
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 20, Issue 21
****************************************
More information about the Histonet
mailing list