[Histonet] liquid nitrogen

manal galal galalmkh <@t> yahoo.com
Sat Jul 16 07:06:46 CDT 2005

Hi all, 
  I wanted to ask about how to use liquid nitogen in freezing muscle biopsies. I mean what does it come in? How much do I use? Do I discard it after I use it? What kind of container do I use? How long do I put the biopsy in it? How do I store it? 
  By the way I freeze muscle in the quick freezing chamber of the cryostat, and am getting very little to no artifacts. But anyone that learns of how I freeeze biopsies says that it will give me disasterous resuls. Do you think liquid nitrogen will be superior to my old method.
Thanks in advance

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