[Histonet] Xyless?

[cfockler <@t> mail1.vcu.edu]

-------------------
> Rich & Andi,
>     Rich, I'd like to second Andi's recommendation for Sigma as a
source 
> for Fast Myosin although we don't bother with HIER. Give it a whirl
both 
> ways, you never know her's might be better :-)
>     Andi, while you asked Rich I figured I'd chime in for Dako as a 
> source for Hepatitis (core & surface). Unfortunately I'm out of the
lab 
> right now so I don't have the cat #s. If  you need them just drop me
a 
> line and I'll rummage them up.
> Good luck guys,
> Amos
> 
>
//////////////////////////////////////////////////////////////////////
////////////////
> 
> Message: 22
> Date: Tue, 12 Jul 2005 16:37:08 +0200
> From: "Andi Kappeler" <kappeler <@t> patho.unibe.ch>
> Subject: Re: [Histonet] Fast myosin IHC
> To: "Richard Cartun" <Rcartun <@t> harthosp.org>,	"Histonet"
> 	<HistoNet <@t> pathology.swmed.edu>
> Message-ID: <008801c586ef$40bf45f0$27955c82 <@t> patho.unibe.ch>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> 	reply-type=original
> 
> Hi Richard
> 
> We successfully use:
> - mo-a-Myosin II (fast), clone MY-32, Sigma M 4276, working conc.
approx 10 
> ug Ig/ml (corresponds to 1:400 with the lot we have), HIER in
citrate pH 6.0 
> (pressure cooker)
> - mo-a-Myosin I (slow), clone NOQ7.5.4D, Sigma M 8421, working conc.
approx 
> 7.5 ug Ig/ml (corresponds to 1:1000 with the lot we have), HIER in
citrate 
> pH 6.0 (pressure cooker)
> Both mAbs work very well (see 
> http://www.pathology.unibe.ch/Diagn/speztech/spez_ihc.htm for a pic
(german 
> website, sorry).
> 
> Can you give me a hint in return, where you get your antibodies
against 
> Hepatitis B surface Ag (HBs) and core Ag (HBc) from? Our source is
no longer 
> able to supply them, due to some bureaucratic / eurocratic rules,
now I'm 
> looking for a reliable replacement.
> Many thanks in advance!
> 
> Best regards
> Andi Kappeler
> Institute of Pathology, University of Bern, Switzerland
> 
> 
> 
> 
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> 
> Hello All . .. I just recently moved to a lab that uses a few
changes of Xyless, (xylene substitute) in their stainer and processor.
 .can anyone give me any information about this product? I can't find
anything useful on the web.  Thanks in advance!


Candyce LeTellier  
Histotechnologist