[Histonet] Ventana Benchmark XT and ALK 1

[Malam Jacqueline]

Does anyone out there do ALK 1 on the Ventana Benchmark? If so, I would be
grateful if you could let me know what clone you use, what the protocol and
dilution are, and if you could spare me a positive control block. 
I have tried 2 clones - Dako's ALK 1 and Lab Vision's SP8 but to no avail; I
have had experience of certain clones of some antisera (Vector's CD 138 and
Dako's calretinin for example) not working on the Benchmark and it's
possible that these clones are not suitable. Having said that, we only had 2
cases and possibly they may have been negative for ALK 1 anyway. I used the
lowest recommended dilution, both the mild and standard heat protocols, and
the 3 primary incubation temperatures (room, 37 and 42) and even tried
protease and no retrieval but got nowhere. I have also tried to get a known
positive control block but no one I've tried has one!
All assistance appreciated!
Jacqui Malam
Royal Infirmary
Lancaster
England



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