[Histonet] overdecalcified bone

Goodwin, Diana GoodwinD <@t> pahosp.com
Thu Jul 14 13:50:36 CDT 2005

We used the 5% periodic acid overnight method with remarkable results.  I am
writing a procedure...do you have any idea why these methods work, ie, the
chemistry behind the method?  I found the original method in the AFIP
manual, but no theory was given.
Diana G. Goodwin
Department of Pathology
Pennsylvania Hospital
800 Spruce St., Preston 655-C
Philadelphia, PA  19107
ph:  215-829-6532
fax:  215-829-7564
e-mail:  goodwind <@t> pahosp.com

----Original Message-----
From: Hermina Borgerink [mailto:hborgeri <@t> wfubmc.edu]
Sent: Wednesday, July 13, 2005 1:40 PM
To: Goodwin, Diana; kalschev <@t> svm.vetmed.wisc.edu
Cc: Histonet (E-mail)
Subject: RE: [Histonet] overdecalcified bone

>From Theory and practice of Histotechnology, Sheehan and Hrapchak, Appendix
B, p. 445.  


To restore basophilic properties to tissues as the result of overexposure to
decalcifying solution:


Method 1

1.    Deparaffinize and hydrate slides

2.    Place slides in 5% aqueous sodium bicarbonate solution from 4 hours to

3.    Wash in tap water for 5 minutes

4.    Stain as desired


Method 2

In step 2, use 5% aqueous solution of ammonium sulfide overnight


Method 3

In step 2, use 5% aqueous solution of periodic acid overnight


Hopefully one of these methods will work for you.





Hermina M. Borgerink, BA, HTL(ASCP)QIHC

Wake Forest University Health Sciences

Department of Pathology

Medical Center Blvd.

Winston-Salem, NC 27157

Tel. (336) 716-1538

Fax. (336) 716-1515

e-mail hborgeri <@t> wfubmc.edu 


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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Goodwin,
Sent: Wednesday, July 13, 2005 1:12 PM
To: 'kalschev <@t> svm.vetmed.wisc.edu'
Cc: Histonet (E-mail)
Subject: [Histonet] overdecalcified bone


Hi, Vicki.


Is there a procedure to restore the nuclear staining uptake in

over-decalcified human bone?


Diana Goodwin

Supervisor, Anatomic Pathology

Pennsylvania Hospital, Preston 655-C


ph:  215-829-6532

fax:  215-829-7564

e-mail:  goodwind <@t> pahosp.com



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