[Histonet] H&E after IHC

Monfils, Paul PMonfils <@t> Lifespan.org
Wed Jul 13 13:40:33 CDT 2005

Hematoxylin is an acceptable counterstain for immunoperoxidase techniques
(though I personally prefer methyl green).  I would avoid eosin however.
The brown color that develops in such techniques has a definite reddish
cast, and using a background stain in the red range could interfere with
interpretation of results, especially in weakly stained areas.

Paul M.

> ----------
> From: 	histonet-bounces <@t> lists.utsouthwestern.edu on behalf of wen
> eng
> Sent: 	Wednesday, July 13, 2005 11:13 AM
> To: 	histonet
> Subject: 	[Histonet] H&E after IHC
> Hi histonetters,
> I have to ask a basic question. I am going to do IHC using DAB. I used to
> do hematoxylin counterstaining. Can I do H&E after IHC? Will it cover
> brown color of DAB? 
> Thanks,
> Wen
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