[Histonet] overdecalcified bone

[Hermina Borgerink]

>From Theory and practice of Histotechnology, Sheehan and Hrapchak,
Appendix B, p. 445.  

 

To restore basophilic properties to tissues as the result of
overexposure to decalcifying solution:

 

Method 1

1.    Deparaffinize and hydrate slides

2.    Place slides in 5% aqueous sodium bicarbonate solution from 4
hours to overnight

3.    Wash in tap water for 5 minutes

4.    Stain as desired

 

Method 2

In step 2, use 5% aqueous solution of ammonium sulfide overnight

 

Method 3

In step 2, use 5% aqueous solution of periodic acid overnight

 

Hopefully one of these methods will work for you.

Hermina

 

 

 

Hermina M. Borgerink, BA, HTL(ASCP)QIHC

Wake Forest University Health Sciences

Department of Pathology

Medical Center Blvd.

Winston-Salem, NC 27157

Tel. (336) 716-1538

Fax. (336) 716-1515

e-mail hborgeri <@t> wfubmc.edu 

 

"Treat a man as he appears to be, and you make him worse.  But treat a
man as if he already were what he potentially could be, and you make him
what he should be."  (Goethe)

 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Goodwin,
Diana
Sent: Wednesday, July 13, 2005 1:12 PM
To: 'kalschev <@t> svm.vetmed.wisc.edu'
Cc: Histonet (E-mail)
Subject: [Histonet] overdecalcified bone

 

Hi, Vicki.

 

Is there a procedure to restore the nuclear staining uptake in

over-decalcified human bone?

 

Diana Goodwin

Supervisor, Anatomic Pathology

Pennsylvania Hospital, Preston 655-C

 

ph:  215-829-6532

fax:  215-829-7564

e-mail:  goodwind <@t> pahosp.com

 

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