[Histonet] overdecalcified bone

Hermina Borgerink hborgeri <@t> wfubmc.edu
Wed Jul 13 12:39:57 CDT 2005

>From Theory and practice of Histotechnology, Sheehan and Hrapchak,
Appendix B, p. 445.  


To restore basophilic properties to tissues as the result of
overexposure to decalcifying solution:


Method 1

1.    Deparaffinize and hydrate slides

2.    Place slides in 5% aqueous sodium bicarbonate solution from 4
hours to overnight

3.    Wash in tap water for 5 minutes

4.    Stain as desired


Method 2

In step 2, use 5% aqueous solution of ammonium sulfide overnight


Method 3

In step 2, use 5% aqueous solution of periodic acid overnight


Hopefully one of these methods will work for you.





Hermina M. Borgerink, BA, HTL(ASCP)QIHC

Wake Forest University Health Sciences

Department of Pathology

Medical Center Blvd.

Winston-Salem, NC 27157

Tel. (336) 716-1538

Fax. (336) 716-1515

e-mail hborgeri <@t> wfubmc.edu 


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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Goodwin,
Sent: Wednesday, July 13, 2005 1:12 PM
To: 'kalschev <@t> svm.vetmed.wisc.edu'
Cc: Histonet (E-mail)
Subject: [Histonet] overdecalcified bone


Hi, Vicki.


Is there a procedure to restore the nuclear staining uptake in

over-decalcified human bone?


Diana Goodwin

Supervisor, Anatomic Pathology

Pennsylvania Hospital, Preston 655-C


ph:  215-829-6532

fax:  215-829-7564

e-mail:  goodwind <@t> pahosp.com



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