[Histonet] ADG & Dysferlin troubles

[Lewis, Sarah]

Hello histoneters,
 
    I could really use any procedural information anyone has on Alpha-dystroglycan (antibody only available thru Upstate) and Dysferlin (antibody from VectorLabs).
 
        I have been attempting the stains for 2months now with no satisfying results. I have tried several different procedures/fixatives/incubation times/dilutions/. I perform a battery of 14 neuromuscular Immunoflourescence, they are all BEAUTIFUL fitc green with no background staining (picture perfect) .....except these two boogers. PLEASE HELP me make my pathologist proud!!! ALL THE CREDIT TO HISTONET!!!!   

Sarah E. Lewis 
Histotechnician 
Childrens Research 
Center for Gene Therapy 
700 Childrens Dr Rm WA3112 
Columbus OH 43205 
(614)-722-2204 
LewisS <@t> ccri.net