[Histonet] Fast myosin IHC

Andi Kappeler kappeler <@t> patho.unibe.ch
Tue Jul 12 09:37:08 CDT 2005

Hi Richard

We successfully use:
- mo-a-Myosin II (fast), clone MY-32, Sigma M 4276, working conc. approx 10 
ug Ig/ml (corresponds to 1:400 with the lot we have), HIER in citrate pH 6.0 
(pressure cooker)
- mo-a-Myosin I (slow), clone NOQ7.5.4D, Sigma M 8421, working conc. approx 
7.5 ug Ig/ml (corresponds to 1:1000 with the lot we have), HIER in citrate 
pH 6.0 (pressure cooker)
Both mAbs work very well (see 
http://www.pathology.unibe.ch/Diagn/speztech/spez_ihc.htm for a pic (german 
website, sorry).

Can you give me a hint in return, where you get your antibodies against 
Hepatitis B surface Ag (HBs) and core Ag (HBc) from? Our source is no longer 
able to supply them, due to some bureaucratic / eurocratic rules, now I'm 
looking for a reliable replacement.
Many thanks in advance!

Best regards
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message ----- 
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, July 12, 2005 3:26 PM
Subject: [Histonet] Fast myosin IHC

> Is anyone doing immunohistochemistry for fast myosin on formalin-fixed 
> human tissue?  If so, whose antibody are you using?  My "Pub Med" search 
> did not turn up much.  Thanks!
> Richard
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 545-1596
> (860) 545-0174 Fax
> -----------------------------------------------
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