[Histonet] Macrophage marker mouse tissue

[Luis Chiriboga]

A couple of years ago, I used (in frozen mouse heart) Serotec (cat#
MCA1957s) rat anti-mouse cd68 which targets macrosialin, the murine CD68
homologue. It's a monoclonal (FA-11) IgG2a, detected  with a biotinylated
rabbit anti-rat (mouse adsorbed) followed by SA-alkaline phosphatase red.
It worked very nicely but at a very high concentration. At the time they had
not determined whether it was viable in FFPE so you may want to give them a
call and see.
Hope this helps
Regards Luis

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of GT Hebert
Sent: Monday, July 11, 2005 12:30 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Macrophage marker mouse tissue


Hello,

I am in need of a good macrophage marker on FFPE tissue.  I will be using it
on mouse tissue... mainly the heart (but other tissues may be included).  So
far I have looked into a number of antibodies (CD68, Mac-2) but can never be
certain which one to choose.

Thank you in advance for your help.

Sincerely,

Gustave



Gustave Hebert, HT (ASCP)
Scientist II
Wyeth Research
Cardiovascular and Metabolic Diseases
Cambridge MA


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