[Histonet] Macrophage marker mouse tissue
Luis.Chiriboga <@t> med.nyu.edu
Mon Jul 11 13:07:34 CDT 2005
A couple of years ago, I used (in frozen mouse heart) Serotec (cat#
MCA1957s) rat anti-mouse cd68 which targets macrosialin, the murine CD68
homologue. It's a monoclonal (FA-11) IgG2a, detected with a biotinylated
rabbit anti-rat (mouse adsorbed) followed by SA-alkaline phosphatase red.
It worked very nicely but at a very high concentration. At the time they had
not determined whether it was viable in FFPE so you may want to give them a
call and see.
Hope this helps
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of GT Hebert
Sent: Monday, July 11, 2005 12:30 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Macrophage marker mouse tissue
I am in need of a good macrophage marker on FFPE tissue. I will be using it
on mouse tissue... mainly the heart (but other tissues may be included). So
far I have looked into a number of antibodies (CD68, Mac-2) but can never be
certain which one to choose.
Thank you in advance for your help.
Gustave Hebert, HT (ASCP)
Cardiovascular and Metabolic Diseases
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