[Histonet] seeking mouse CD31 and mouse CD4 on FFPE

Koelling, Ray koellinr <@t> amgen.com
Fri Jul 8 12:59:00 CDT 2005

We use Pham BD anti-CD31 (MEC 13.3 clone) on FFPE mouse tissue.

5 min Dako Proteinase K
Overnight in primary in refrigerator at 1 to 20,000
Biotin labeled secondary
5 min TSA amplification

Works very nicely and if you come to nationals in Florida, there will be a
photomicrograph of the stain on our automated IHC poster.

There will also be a picture of anti-mouse CD4 on FFPE mouse tissue for
anyone interested in that elusive goal.


 -----Original Message-----
From: 	histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]  On Behalf Of Christian
Sent:	Friday, July 08, 2005 10:19 AM
To:	histonet <@t> lists.utsouthwestern.edu
Subject:	[Histonet] seeking mouse CD31 Ab

I've tried many and none seem to work with FFPE sections.  Human Cd31 
isn't a problem at all, even in FFPe sections but,  mouse is a 
different story.
Anyone out there has had success in staining mouse vessels in formalin 
sections?  Any good Ab's or tricks?

thanks in advance.
Happy Friday!


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