[Histonet] seeking mouse CD31 and mouse CD4 on FFPE
koellinr <@t> amgen.com
Fri Jul 8 12:59:00 CDT 2005
We use Pham BD anti-CD31 (MEC 13.3 clone) on FFPE mouse tissue.
5 min Dako Proteinase K
Overnight in primary in refrigerator at 1 to 20,000
Biotin labeled secondary
5 min TSA amplification
Works very nicely and if you come to nationals in Florida, there will be a
photomicrograph of the stain on our automated IHC poster.
There will also be a picture of anti-mouse CD4 on FFPE mouse tissue for
anyone interested in that elusive goal.
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Christian
Sent: Friday, July 08, 2005 10:19 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] seeking mouse CD31 Ab
I've tried many and none seem to work with FFPE sections. Human Cd31
isn't a problem at all, even in FFPe sections but, mouse is a
Anyone out there has had success in staining mouse vessels in formalin
sections? Any good Ab's or tricks?
thanks in advance.
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