[Histonet] PCNA on bone

[Pixley, Sarah (pixleysk)]

Dear Lin:
If you are having trouble with PCNA on RAT decalcified bone, then your
problem might be the decalcification procedure. We just went through a lot
of trouble to learn that BrDU staining does not work on tissue treated with
our rapid decalcifying solution(unknown acid decalcification). Instead, we
needed either EDTA or formic acid decalcification to get it to work. But we
did previously do PCNA staining using this rapid decalcifier and got great
staining with Chemicon mouse MAB424 PCNA. 

If you are trying to stain mouse tissues then you have a different and
bigger can of worms. We could not get it to stain and heard from others that
you needed a MOM (mouse on mouse) kit to get it to work. I will be
interested to hear if there is a PCNA antibody that works well on mouse
tissues. 

Sarah