[Histonet] slippery pig snouts

[Elizabeth Chlipala]

Trisha

We have stained a lot of cartilage, not pig snots, but this might be
helpful.  We cut our slides and let them drain a bit and then place them
flat on a hot plate overnight.  The hot plate is not to hot so that the
paraffin melts.  We do not place them in a hot oven, we find that the
cartilage might flip.  We deparaffinize and then check if any of the
cartilage is out of place.  You can then dip the slides in water and
gently get the cartilage to go back in place.  If I'm having a difficult
time with the sections falling off I would allow them to dry flat before
I begin the immunostaining procedure. 

I hope this helps.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Trisha
Emry
Sent: Thursday, July 07, 2005 1:24 PM
To: histo
Subject: [Histonet] slippery pig snouts

Hi,

We are trying to do brdu on nasal septums of pig snouts.
I put them on plus coated slides and they are falling off during the
process.

Have any of you found a way to keep cartilage on slides?
How long and at what temperature can we heat the slides to see if that
will
help?

Thanks,

Trisha
Seattle


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