[Histonet] CK17

[Elaine Dooley]

Dear Histonetters,

I have been trying to get CK17(M7046) from DAKO to work on the Ventana
Benchmark.  I have tried several different tissues for controls.  I have
several dilutions and tried several types of retrieval.  I get staining
but I also get background that does not seem to dilute out.  I have
tried DAKO's background reducing diluent and it helps somewhat.

If anyone uses this antibody on the Ventana Benchmark I wonder if you
would share your protocol?  What type of control tissue do you
recommend? What dilution do you use? 

For those who use other stainers is there a particular pretreatment
that you recommend?

Thanks in advance

Elaine Dooley