[Histonet] Enzymatic digestion

[Geoff McAuliffe]

The answer to this question will depend on the tissue, fixative used, 
time of fixation, antigen in question, antibody used, and probably other 
factors as well. A literature search is in order.

Geoff

Lab. Invest. Histol. wrote:

>I would need to know the differences in the effect (degree of digestion) of
>each one of these enzymes used for antigen retrieval.
>
>Proteinase K
>Ficin
>Pepsin
>Tripsin
>
> 
>Thank you
>
>         Hugo
>
>-------------------------------------------------------------------
>Dr. Hugo H. Ortega (DMV, PhD)
>Departament of Cellular Biology
>Faculty of Veterinary Sciences
>Universidad Nacional del Litoral
>
>R.P. Kreder 2805 - Esperanza (3080)
>Santa Fe - ARGENTINA
>Tel. (54)3496-420639
>Fax. (54)3496-426304
>http://fcv.unl.edu.ar/histolog/
>http://fcv.unl.edu.ar/bioterio/
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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