[Histonet] RE: Histonet Digest, Vol 20, Issue 7

Wed Jul 6 14:00:03 CDT 2005

I have the Shandon senior, it is a very difficult unit to clean and keep clean as well as having mechanical problems to the extent that Shanon agreed to extend the warranty for an additional year. My opinion is that is over engineered, too much electro mechanical "stuff"
mike rice
ft lauderdale

-----Original Message-----
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histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, July 06, 2005 1:31 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 20, Issue 7

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Today's Topics:

   1. RE:no nuclei in frozen sections Last post wrong subject
      (Stephen Peters M.D.)
   2. Job Opening (Janice A Mahoney)
   3. (no subject) (Nava, Josefa)
   4. Urgent feedback on MOPEC MB600 VS. Shandon Grosslab	Senior 
      (Nava, Josefa)
   5. microm (Richard Allan) v. Leica glass coverslipper
      (Goodwin, Diana)
   6. HistoWiki - The Free Histology Encyclopedia - Launched!
      (IHC World)
   7. goat anti-galectin 1 (Patsy Ruegg)
   8. RE: goat anti-galectin 1 (Elizabeth Chlipala)

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Message: 1
Date: Wed, 6 Jul 2005 07:20:31 -0700 (PDT)
From: "Stephen Peters M.D." <petepath <@t> yahoo.com>
Subject: [Histonet] RE:no nuclei in frozen sections Last post wrong
	subject
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <20050706142031.88609.qmail <@t> web30412.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Are the nuclei totally missing? or are there holes in the nuclei? If there are holes in the nuclei 
I believe this is one of the manifestations of freeze artefact which becomes very obvious 
in sections in the under 3 micra range. Even though a cryostat is set for 5 micra it is very
 common for the first few sections to be different thickness, commonly a thick one followed 
by a thin one. 
I show pics of what I believe are nuclear ice crystals and the effect of section thickness on
 this page of my frozen section tutorial:  http://pathologyinnovations.com/new_page_3.htm.

If the nuclei are falling out, I have no answer.

Stephen

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Message: 2
Date: Wed, 06 Jul 2005 09:40:40 -0500
From: "Janice A Mahoney" <jmahoney <@t> alegent.org>
Subject: [Histonet] Job Opening
To: <histonet <@t> pathology.swmed.edu>
Message-ID: <s2cba721.058 <@t> mail.alegent.org>
Content-Type: text/plain; charset=US-ASCII

Histology Technicians
Full Time/Casual/On-call/Flexible shifts available

Alegent Health is currently looking for a Histology Tech.
Registered by American Society of 
Clinical Pathologists or eligible.  Two years experience
in a clinical laboratory preferred.
Alegent (logo)

Apply on-line:www.alegent.com
Pre-employment physical, drug screen and background check required prior to hire.
AA/EEO

Jan Mahoney
Omaha NE

------------------------------

Message: 3
Date: Wed, 6 Jul 2005 10:17:58 -0500
From: "Nava, Josefa" <JosefaNava <@t> texashealth.org>
Subject: [Histonet] (no subject)
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<2C515C1049EAF5459EFD8C9B929078A41944BF <@t> phdex03.txhealth.org>
Content-Type: text/plain;	charset="us-ascii"

We are looking to buy a new grossing station and are deciding between
the Shandon Grosslab Senior   vs. the MOPEC MB600. Has anyone used the
MOPEC MB600 and what do you like/ dislike about  it? I thank you for any
feedback you can

 give  us.

Josie Nava

Presbyterian Hospital of Dallas

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------------------------------

Message: 4
Date: Wed, 6 Jul 2005 10:27:10 -0500
From: "Nava, Josefa" <JosefaNava <@t> texashealth.org>
Subject: [Histonet] Urgent feedback on MOPEC MB600 VS. Shandon
	Grosslab	Senior 
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<2C515C1049EAF5459EFD8C9B929078A41944C0 <@t> phdex03.txhealth.org>
Content-Type: text/plain;	charset="us-ascii"

We  are looking to buy a new  grossing station and are deciding between
the Shandon Grosslab Senior vs. the MOPEC MB600. Has anyone used the
MOPEC MB600 and  what do you like about it? I thank you for any
information you can give us. 

Josie Nava

Presbyterian Hospital of Dallas

The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are prohibited from copying, distributing, or using the information.  Please contact the sender immediately by return e-mail and delete the original message from your system.

------------------------------

Message: 5
Date: Wed, 6 Jul 2005 11:38:44 -0400 
From: "Goodwin, Diana" <GoodwinD <@t> pahosp.com>
Subject: [Histonet] microm (Richard Allan) v. Leica glass coverslipper
To: "Histonet (E-mail)" <HistoNet <@t> Pathology.swmed.edu>
Message-ID:
	<992899E9EC268548AB8DDE246AF88473055F51E0 <@t> PAHEX01.uphs.upenn.edu>
Content-Type: text/plain;	charset="iso-8859-1"

Soliciting comments, pro and con, for these two instruments.

Thanks!

Diana Goodwin
Supervisor, Anatomic Pathology
Pennsylvania Hospital, Philadelphia
ph:  215-829-6532
fax:  215-829-7564
e-mail:  goodwind <@t> pahosp.com

------------------------------

Message: 6
Date: Wed, 6 Jul 2005 11:50:25 -0400
From: "IHC World" <info <@t> ihcworld.com>
Subject: [Histonet] HistoWiki - The Free Histology Encyclopedia -
	Launched!
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <NEEPIKCBDDLLFFPGHNFGCEIPCNAA.info <@t> ihcworld.com>
Content-Type: text/plain;	charset="iso-8859-1"

Hello All,

Everyone on the Histoland is invited to participate the histology project -
HistoWiki - the free-content histology encyclopedia that anyone can edit.
The goal of setting up this site is to build the world largest, the most
comprehensive and up-to-date encyclopedia about everything related to
Histology.

http://www.ihcworld.com/histowiki/

If you have any questions or suggestions, please drop me a email at
info <@t> ihcworld.com

Thanks.

Richard
IHC World
Email: info <@t> ihcworld.com

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Message: 7
Date: Wed, 6 Jul 2005 09:54:38 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: [Histonet] goat anti-galectin 1
To: "'Histonet'" <histonet <@t> pathology.swmed.edu>
Message-ID: <200507061554.j66FsckM006052 <@t> chip.viawest.net>
Content-Type: text/plain;	charset="us-ascii"

Anybody using SC goat anti-galectin 1 antibody on ffpe tissue? If so please
share your protocol.

Thank you,

Patsy

P.S.

Has anyone developed a labelled polymer type detection system to use with
goat primary antibodies yet?

------------------------------

Message: 8
Date: Wed, 6 Jul 2005 10:07:17 -0600
From: "Elizabeth Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] goat anti-galectin 1
To: "'Patsy Ruegg'" <pruegg <@t> ihctech.net>,	"'Histonet'"
	<histonet <@t> pathology.swmed.edu>
Message-ID: <000001c58244$c7f20080$a7d48a80 <@t> AMY>
Content-Type: text/plain;	charset="us-ascii"

Patsy

I use labeled polymers with goat primaries as follows:

After the goat antibody I use a rabbit anti-goat secondary.  I get this
from vector labs and then I use the rabbit envision + next.  It works
great.  

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com

Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Wednesday, July 06, 2005 8:55 AM
To: 'Histonet'
Subject: [Histonet] goat anti-galectin 1

Anybody using SC goat anti-galectin 1 antibody on ffpe tissue? If so
please
share your protocol.

Thank you,

Patsy

P.S.

Has anyone developed a labelled polymer type detection system to use
with
goat primary antibodies yet?

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End of Histonet Digest, Vol 20, Issue 7
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