[Histonet] no nuclei in frozen sections

[Snider, Deanna]

Hello again everyone!  
I need your advice and suggestions again!  I have begun to have holes in
frozen sections where the nuclues should be.  I am cutting clinically
engineered skin specimens, at -21.  NO flash freeze spray.  The specimens
are cut at 5-6 microns and are embedded in Shandons' M-1 embedding matrix.
This problem seems to have come up in the last 3 weeks or so, but I cannot
unravel why.  Nothing has been changed on my end, but the problem has been
in all the researchers submissions.  I have done recuts and they are still
there.  I have wracked my brain and am coming up clueless!  I have contacted
the manufacturer for additional information on the properties of M-1, but I
am still coming up with no answers.  Thanks in advance,
Deanna
Shriners Hospital for Children
Research Dept.
Cincinnati, Oh
513-872-6388

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