[Histonet] Plus slides again

Steven Coakley sjchtascp <@t> yahoo.com
Fri Jul 1 14:24:16 CDT 2005

I've tested 4 different brands of plus slides and they all pretty much react the same.  Pick the section up, wait a few seconds and float it right off the slide.  I know there not working well because I see significant tissue loosening of my sections after staining.  I've used plus slides at other facilities w/o being able to float off the section.  I'm using distilled, about pH 6 in my water bath, no adhesive.  Temp about 46-48C.  Float the ribbon out, let them flatten for a few seconds, pick them up and vertically let them air dry completely before drying them at 60-65C in a forced air dryer.  Elementary problem but rather annoying.

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