Hi, What duration of fixation is recommanded for bonemarrow trephines (3 mm diameter), if you use a formicacid-formaldehyd solution subsequently for decalcification? Please, can anyone share his/her experience in this field. (morphology, ihc) Our protocol: ca. 20 hours 9% buffered formaldehyd; then 6-8 hours decal with the combinated solution. Thank you Gudrun