[Histonet] Recipe for 4%PFA w/saponin

[vsailes <@t> nd.edu]

Hi Histonetters,
I'm working with mice and we want to stain the livers for albumin. I have an
antibody that appears to work but we would like to perfect the technique if
possible. I have done some literature searches and some of them suggested that
to perfuse the animal with 4% paraformaldehyde containing saponin would give a
more homogenous staining pattern but I really could not find information
telling the percentage of saponin to use.
If anyone out there has experience with this would you please help shed some
light on this matter.
Thank you in advance for your help.
Have a great holiday weekend.
Valerie