[Histonet] Re: endogenous peroxidase blocking in acetone fixed preps

[C.M. van der Loos]

   Dear Brian,

   The solution as you suggested works fine indeed for abolishing
   peroxidase activity in erythrocytes. However, it kills the endogenous
   peroxidase activity in neutrophils just marginally.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academical Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631
   fax:    +31 20 6960389
   e-mail: [1]c.m.vanderloos <@t> amc.uva.nl


   ----- Original Message -----
   From  Brian Chelack <BRIAN.CHELACK <@t> usask.ca>
   Date  Fri, 28 Jan 2005 17:31:31 -0600
   To  Histonet <@t> Pathology.swmed.edu
   Subject  [Histonet] Re: endogenous peroxidase blocking in acetone
   fixed preps.

   I have had success using the following treatment:
   1% H2O2
   0.1% Na Azide
   in PBS
   add to acetone fixed section/cell prep/smear for 10-12
   minutes
   rinse 3X with PBS
   Carry on with blocking, primary antibody etc.
   regards,
   Brian Chelack

References

   1. mailto:c.m.vanderloos <@t> amc.uva.nl