[Histonet] HRP-conjugated secondary and biotinylated-secondary

Subratab subratab <@t> bdonline.com
Sat Jan 29 12:18:56 CST 2005


   Hi,
   Is it a common practice to use HRP-conjugated secondary antibody
   instead of biotinylated secondary antibody for immunohistochemistry
   (with DAB developing system)? I am confused about the advantage/
   disadvantage of using HRP-conjugated secondary antibody for immunos.
   Subrata Biswas
   UNICAMP, SP, Brazil.



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