[Histonet] Re: endogenous peroxidase blocking in acetone fixed preps.

Brian Chelack BRIAN.CHELACK <@t> usask.ca
Fri Jan 28 17:31:31 CST 2005


I have had success using the following treatment:

1% H2O2 
0.1% Na Azide
in PBS 

add to acetone fixed section/cell prep/smear for 10-12
minutes

rinse 3X with PBS 

Carry on with blocking, primary antibody etc.


regards,
Brian Chelack




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