[Histonet] bubbles

[Robyn Vazquez]

Danielle,
Did you make the glycerol fresh or make it and let it set over night? 
I do immunofluorescence and use a 1:10 glycerol/pbs solution.  Could you
use the same?
 
Robyn
OHSU

>>> Danielle Jin <zhuxx003 <@t> umn.edu> 1/24/2005 2:05:21 PM >>>

Hello,

I am new to histonet. I hope someone out there can help me solve my
problem.   When I do muscle staining (eg. acid phosphatase), the last
step is use glycerol Gelatin to cover the coverslip.  Somehow it
always
trap some bubbles in the tissue section.  How can I get rid of the
bubbles?  Thanks

Danielle Jin



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