[Histonet] endogenous alk phos

[Gayle Callis]

What tissues are you working with?  Gut?

There is a blocker from KPL, a universal blocker that will work, but you 
use it at beginning of staining, as one uses peroxidase  blocking.   It 
also blocks endog peroxidase.  I am not sure what it will do to frozen 
sections - a shorter time for application,  but they say it can be used 
with frozen sections.  It is wonderful with paraffin sections and if one 
uses this blocker, you do NOT use levamisole in the chromogen.

The reagent is ready to use, we just recycle dropper bottles for 
application of drops.



At 08:37 AM 1/18/2005, you wrote:
>Hello. Is there another method(s) to block endogenous alkaline phosphotase
>in mice, on frozen sections, besides levamisole?
>
>Thanks!!!!!
>
>Rachael L. Emerson
>Center for Human Genetics and Molecular Pediatric Diseases
>University of Rochester Medical Center
>575 Elmwood Avenue MRBX 1.11301
>Rochester, NY 14642
>
>Tel (585) 275-5073
>Fax (585) 276-0232
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)