[Histonet] Azur B

[Rittman, Barry]

As far as I remember the sequence is methylene blue, azure B, azure A, azure 4, azure C, Bernthen's methylene violet and thionin. This is a progression and,  in general the dyes having greater metachromatic properties the closer that they are to thionin and pH dependent.
Barry

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Patsy Ruegg
Sent: Tue 1/11/2005 10:21 AM
To: 'Kristopher Kalleberg'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Azur B



I use Azure B as a proteoglycan stain, it will stain different tissue
components depending on the ph.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kristopher
Kalleberg
Sent: Tuesday, January 11, 2005 6:39 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Azur B

HAs anyone used Azur(e) B for a melanin stain.  I read the other day that
you can use Azur B to stain malanin a green-blue, but everything else I read
only says it is used for plant tissue.  If anyone has used it for melanin,
how does it work and what would be the best counterstain.  Thanks.

Kris L Kalleberg
Unilever R&D
45 River Rd.
Edgewater, NJ 07020
201 840 2472


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