[Histonet] oil red in MMA

[John Kiernan]

Embedding in butylmethacrylate and methylmethacrylate
involves passage through solvents (alcohol or
acetone) and the liquid methacrylate monomers.
All these liquids dissolve fat and other
hydrophobic lipids, and the sections will not
contain material stainable with oil red O.
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John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
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Myri37 <@t> aol.com wrote:
> 
> Hi dear histonnetters and happy new year 2005 !!
> does anyone have a protocol of oil red staining in tissu embedded in MMA (mixture of butylmetacrylate and methylmetacrylate)?
> i would like to stain fats in soft tissu on titane. My sections are 50Um thikness.
> thank you in advance
> Myriam