[Histonet] H&E staining on GMA plastic

[Flynn, Evelyn]

Dear Geoff and others,
      This is the protocol that I use.

H&E of JB-4 (Plastic) Sections

DO NOT USE XYLENE OR OTHER ORGANIC SOLVENTS

1. Harris Hematoxylin, 12 minutes.
2. Rinse in running tap water until clear.
3. Quick dip in eosin.
4. Rinse in running tap water, 10 minutes
5. Air dry
6. Coverslip with Cytoseal or other mounting medium.

Evelyn Flynn
Children's Hospital, Boston


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Geoff McAuliffe
Sent: Thu 1/6/2005 7:00 PM
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E staining on GMA plastic
 
Dear List

    A colleague wants to do some H and E staining on zebrafish embryos 
embedded in JB4+ plastic. Does anyone have a good protocol they can 
share? Thanks in advance!

Geoff
--

**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
**********************************************




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