[Histonet] rescue sections for staining
J. Douglas Swarts
dswarts <@t> pitt.edu
Fri Feb 25 12:35:48 CST 2005
We are trying to stain some sections cut about ten years ago. They are
rat cranial bases including the middle ear which is the area we are
interested in. When we stain the sections with H&E we get no
hematoxylin staining, ie. no nuclei at all, but the eosin gives a nice
uniform pink color.
The specimens were decalcified in 10% formic acid which was neutralized
with sodium sulfate according to the histologists notes. The sections
stained at the time show very little hematoxylin staining. So the
Are these sections overdecalcified? If so is there any technique I can
use to rescue them?
Did the neutralization with sodium sulfate create the problem? If so
can I do anything to reverse the effect?
Finally, if one or the other of these situations are true and cannot be
remedied is there another staining protocol that will give me a
"reasonable" level of morphologic detail?
Thanks for your help.
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