[Histonet] xylene free mounting media

Smith, Allen asmith <@t> mail.barry.edu
Fri Feb 25 08:16:00 CST 2005

If one sticks to stains that are fast in absolute alcohol, one can mount
from absolute alcohol in Euparal (gum sandarac), which uses eucalyptol as a
solvent.  Hematoxylin fades slowly in Euparal, but is said to keep virtually
forever in Euparal Vert.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robin
Sent: Monday, February 21, 2005 8:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] xylene free mounting media

We have a tech in our lab who is sensitive to xylene (exhibits headaches,
puffy eyes and itchy skin).  We are switching to Formula 83 by CBG Biotech
for frozen sections but still need a mountant that is both compatible with
this and xylene free.  Does anyone have any suggestions?  

Robin Maxwell-Nunn, MLT
St Mary's General Hospital,
Kitchener, Ontario, Canada.

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